Publications related to Design of Highly Functional Genome Editors by Modelling Crispr-cas Sequences

Related publications (10)

Comparison of Three Viral Nucleic Acid Preamplification Pipelines for Sewage Viral Metagenomics

Viral metagenomics is a useful tool for detecting multiple human viruses in urban sewage. However, more refined protocols are required for its effective use in disease surveillance. In this study, we investigated the performance of three different preampli ...

2024

Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds

Gordanna Pistoletti Blanchet, Aleksandra Radenovic, Georg Fantner, Sanjin Marion, Vytautas Navikas, Samuel Mendes Leitão, Barney Frederick Drake, Simon Finn Mayer, Helena Miljkovic

In current nanopore-based label-free single-molecule sensing technologies, stochastic processes influence the selection of translocating molecule, translocation rate and translocation velocity. As a result, single-molecule translocations are challenging to ...

2023

Artificial intelligence for natural product drug discovery

Philippe Schwaller, Albert Hofstetter, Michael Alexander Skinnider, Florian Huber

Developments in computational omics technologies have provided new means to access the hidden diversity of natural products, unearthing new potential for drug discovery. In parallel, artificial intelligence approaches such as machine learning have led to e ...

Nature Portfolio2023

Stoichiometry of the Gene Products From the Tetrachloroethene Reductive Dehalogenase Operon pceABCT

Christof Holliger, Julien Maillard, Adrian Schmid, Lorenzo Cimmino

Organohalide respiration (OHR) is a bacterial anaerobic process that uses halogenated compounds, e.g., tetrachloroethene (PCE), as terminal electron acceptors. Our model organisms are Dehalobacter restrictus strain PER-K23, an obligate OHR bacterium (OHRB) ...

Frontiers Media SA2022

Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools

Henning Paul-Julius Stahlberg, Dongchun Ni

Type I CRISPR-Cas systems typically rely on a two-step process to degrade DNA. First, an RNA-guided complex named Cascade identifies the complementary DNA target. The helicase-nuclease fusion enzyme Cas3 is then recruited in trans for processive DNA degrad ...

CELL PRESS2022

Where are the missing gene defects in inherited retinal disorders? Intronic and synonymous variants contribute at least to 4% of CACNA1F-mediated inherited retinal disorders

Daniel Francis Schorderet, Vincent Meyer

Inherited retinal disorders (IRD) represent clinically and genetically heterogeneous diseases. To date, pathogenic variants have been identified in similar to 260 genes. Albeit that many genes are implicated in IRD, for 30-50% of the cases, the gene defect ...

WILEY2019

ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer

Didier Trono, Bart Deplancke, Julien Léonard Duc, Riccardo Dainese, Marco Cassano, Julie Marie Russeil, Michaël Imbeault, Wanze Chen, Suk Min Jang, Carine Delattre-Gubelmann, Petra Catalina Schwalie, Sunil Kumar Raghav

Krüppel-associated box zinc finger proteins (KZFPs) constitute the largest family of mammalian transcription factors, but most remain completely uncharacterized. While initially proposed to primarily repress transposable elements, recent reports have revea ...

Nature Research2019

DNA repair protein Rad18 restricts LINE-1 mobility

Priscilla Turelli

Long interspersed element-1 (LINE-1, L1) is a mobile genetic element comprising about 17% of the human genome. L1 utilizes an endonuclease to insert L1 cDNA into the target genomic DNA, which induces double-strand DNA breaks in the human genome and activat ...

Nature Research2018

KAP1 controls endogenous retroviruses in embryonic stem cells

Hillary Layard-Liesching, Didier Trono, Jacques Rougemont, Daniel Constam, Sonia Verp, Séverine Reynard

More than forty per cent of the mammalian genome is derived from retroelements, of which about one-quarter are endogenous retroviruses (ERVs). Some are still active, notably in mice the highly polymorphic early transposon (ETn)/MusD and intracisternal A-ty ...

Nature Publishing Group2010

A stable system for the high-titer production of multiply attenuated lentiviral vectors

Didier Trono, Romain Zufferey

Lentiviral vectors open exciting perspectives for the genetic treatment of a wide array of inherited and acquired diseases, owing to their ability to govern the efficient delivery, integration, and long-term expression of transgenes into nondividing cells ...

2000