Publication

Refractive Index Tomography by Digital Holographic Microscopy

Christian Depeursinge, Tristan Colomb
2008
Conference Papers

Abstract

3D Refractive Index (RI) tomography appears as a challenging perspective in the observation of microscopic 3D objects, biological cells in particular. Recent developments in Digital Holographic Microscopy have permitted to achieve accurate RI 3D images.

Official source

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Christian Depeursinge, Tristan Colomb
2008
Conference Papers

Abstract

Official source

Related concepts (27)

Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image.

Refractive index

In optics, the refractive index (or refraction index) of an optical medium is a dimensionless number that gives the indication of the light bending ability of that medium. The refractive index determines how much the path of light is bent, or refracted, when entering a material. This is described by Snell's law of refraction, n1 sin θ1 = n2 sin θ2, where θ1 and θ2 are the angle of incidence and angle of refraction, respectively, of a ray crossing the interface between two media with refractive indices n1 and n2.

Birefringence

Birefringence is the optical property of a material having a refractive index that depends on the polarization and propagation direction of light. These optically anisotropic materials are said to be birefringent (or birefractive). The birefringence is often quantified as the maximum difference between refractive indices exhibited by the material. Crystals with non-cubic crystal structures are often birefringent, as are plastics under mechanical stress.

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Refractive Index Tomography by Digital Holographic Microscopy

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