Heat flux as an on-line indicator of metabolic activity in pilot scale bioreactor during the production of Bacillus thuringiensis var. galleriae-based biopesticides

The effective biotechnol. process development depends on the system for monitoring the activity of the culture during cultivation. The feasibility of monitoring the metabolic flux during the cultivation of Bacillus thuringiensis var. galleriae (Btg) in pilot scale bioreactor was studied. All possible sources of heat generation and transfer in the system were accounted to det. the metabolic heat flux caused by the culture alone. Successful demonstration of the heat measurements has revealed the scale-up characteristics of microbial process based on the heat generation data. In addn., influence of complex media sources on heat flux, sporulation and insecticidal crystal protein (ICP) synthesis was understood. Nature of the microbial process has necessitated fed-batch cultivation with glucose soln. feeding in proportions to the metabolic heat prodn. rate and subsequent optimization of the feeding glucose soln. concn. had resulted in an enhanced ICP concn. As a significant outcome, heat flux into the cooling water for the maintenance of bioreactor temp. was found to be in definite correlation with the metabolic heat prodn. rate. This allowed an opportunity for understanding the metabolic status of the culture with continuous online monitoring of the temp. profile of the cooling water in industrial scale fermn. with cost effectiveness. Continuous assessment of cultures through heat flux could be exploited by enhancing the desired metabolic process(s) in industrial scale fermn. [on SciFinder (R)]