Superresolution with Optically-Motivated Blind Deconvolution

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Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

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The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...

With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...

Time-correlated single photon counting techniques have recently established themselves in numerous fields of research. In particular, for fluorescence lifetime imaging, time-correlated single photon counting is often considered the absolute reference in te ...

In this thesis new imaging approaches for optical microscopy are proposed and studied. They are based on the use of dynamic structured illumination in combination with a demodulation detection concept employing CMOS image detectors. Two particular implemen ...

Superresolution with Optically-Motivated Blind Deconvolution

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Structural and Functional Stochastic Super-Resolution Microscopy

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Single Molecule Sensing

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Single molecule detection and fluorescence correlation spectroscopy on surfaces

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Super-resolution fluorescence microscopy based on physical models

The Colored Revolution of Bioimaging

Single-photon detector arrays for time-resolved fluorescence imaging

Application of CMOS image sensors in optical sectioning and polarization microscopy