Protein synthesis - computational modeling and experimental integration

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The chemical master equation (CME) is a system of ordinary differential equations that describes the evolution of a network of chemical reactions as a stochastic process. Its solution yields the probability density vector of the system at each point in time. Solving the CME numerically is in many cases computationally expensive or even infeasible as the number of reachable states can be very large or infinite. We introduce the sliding window method, which computes an approximate solution of the CME by perfo

Recombinant protein-based biologics are increasingly relevant in the pharmaceutical industry. Currently, mammalian cell-based bioprocesses are a routine manufacturing procedure for complex proteins such as recombinant antibodies, and the number of new biologics produced by mammalian cells is progressively increasing. In addition, a growing number of proteins need to be rapidly screened to identify new candidates for clinical trials. To help fill this need, rapid mammalian-based bioprocesses have been establ

For animals as well as for humans, the hypothesis of multiple memory systems involved in different navigation strategies is supported by several biological experiments. However, due to technical limitations, it remains difficult for experimentalists to elucidate how these neural systems interact. We present how a computational model of selection between navigation strategies can be used to analyse phenomena that cannot be directly observed in biological experiments. We reproduce an experiment where the rat'

Proper regulation of cell division is crucial to most organisms. Asymmetric division and unequal cleavage are one of the many mechanisms by which spatial regulation of cell division might be achieved. Indeed, by varying its polarity cues or by modulating the size of its daughters, a cell can potentially influence its cellular neighborhood and possibly act upon it. In C. elegans, asymmetric cell division occurs at early stages of development, and is induced by an asymmetric positioning of the spindle during

This paper presents a solution to the Simultaneous Localization and Mapping (SLAM) problem in the stochastic map framework for a mobile robot navigating in an indoor environment. The approach is based on the concept of the relative map. The idea consists in introducing a map state, which only contains quantities invariant under translation and rotation. This is done in order to have a decoupling between the robot motion and the landmark estimation and therefore not to rely the landmark estimation on the unm

The in vivo and in vitro labeling of fusion proteins with synthetic mols. capable of probing and controlling protein function has the potential to become an important method in functional genomics and proteomics. We have recently introduced an approach for the specific labeling of fusion proteins, which is based on the generation of fusion proteins with the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT) and the irreversible reaction of hAGT with O6-benzylguanine derivs. Here, we report

The overall topic of the investigation was the sepn. of basic proteins by cation exchange displacement chromatog. For this purpose two principal column morphologies were compared for the sepn. of RNase A and a-chymotrypsinogen, two proteins found in the bovine pancreas. These were a column packed with porous particles (Macro-Prep S, 10 mm, 1000 .ANG.) and a monolithic column (UNOTM S1). Both columns are strong cation exchangers, carrying -SO3--groups linked to a hydrophilic polymer support. Poly(diallyl-dim

The original TCV control system was designed in the late 1980s to be inherently network based in that all control operations were performed from X-windows terminals and the control computers were networked. Using VMS-VAX technology, the acquisition system (CAMAC) and control system (BITBUS) were connected to controllers situated in the machine bus of these machines that were linked homogeneously by the VMS 'cluster' technology. Although the computing power and network bandwidth have improved considerably ov

Cross-correlation between the fluctuations of 13Ca-Ha interactions affects the relaxation behavior of two-spin coherences (zero- and double-quantum coherences) involving the 13Ca nuclei of two successive amino acid residues. The cross-correlation rates are shown to depend on a dihedral angle S defined by two planes subtended by the atoms {Ha(i-1),13Ca(i-1),13Ca(i)} and {13Ca(i-1),13Ca(i),Ha(i)}. This dihedral angle is related to the secondary structure of a protein, and can be used as a constraint in variou

This paper suggests several approaches for determining the transient stability of a power network by using an analog VLSI chip for simulating the system behavior. The main advantages of using this method are the much shorter computation time and lower complexity compared to the existing methods that are based on numerical calculations or discrete analog emulators

The effect of melittin on lipid order in dimyristoylphosphatidylcholine bilayers was investigated by Raman spectroscopy and fluorescence anisotropy with diphenylhexatriene as fluorescent probe. In the fluid lipid phase, the Raman results indicated a slight increase in the conformational order of the lipid chains, and the fluorescence anisotropy results indicated a considerable increase in the rigid-body orientational order of the lipid chains. These results were contrasted with the reported decrease in the

Protein synthesis - computational modeling and experimental integration

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Solving the chemical master equation using sliding windows

Transient Gene Expression in Mammalian Cells: Promises and Challenges for Medical Biotechnology

Analyzing Interactions Between Navigation Strategies Using a Computational Model of Action Selection

Characterization of RGS-7 as a regulator of forces involved in C. elegans early cell divisions

SLAM with Corner Features Based on a Relative Map

Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro

Investigation of particle-based and monolithic columns for cation exchange protein displacement chromatography using poly(diallyl-dimethylammonium chloride) as displacer

Evolution not revolution in the TCV tokamak control and acquisition system

Relative Orientation of CaHa-Bond Vectors of Successive Residues in Proteins through Cross-Correlated Relaxation in NMR

On-chip transient stability simulator, 104 times faster than real time

Unifying description of the effect of membrane proteins on lipid order. Verification for the melittin/dimyristoylphosphatidylcholine system