Publications related to Individual cells immobilization for water-borne pathogen detection and enumeration

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Georges Muller, Jean-Baptiste Bureau, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

Philippe Renaud, Harald Van Lintel, Jonathan Bastien Cottet

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3 ...

2019

Impedance Spectroscopy for Single Cell Analysis and Dispensing

David Vincent Bonzon

Flow cytometry is an essential tool in biology and medicine with applications in immunodeficiency diagnostic, immunosurveillence, organ transplantation in addition to its basic research usage. Before the widespread use of the Fluorescent-Activated Cell Sor ...

EPFL2017

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Raphaël Tornay, Nina Buffi, Niccolo Piacentini

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

2011

Analytical expression for electric filed between two facing strip electrodes in microchannels

Philippe Renaud, Urban Seger, Olof Pontus Linderholm

An analytical solution is presented for the electric field between two facing strip electrodes situated in a microchannel, as frequently used in impedance cytometry applications. The measured change in resistance as induced by a 5 mm bead moving through th ...

2006