Publication

Single-molecule avidin-biotin association reaction studied by force-clamp spectroscopy

Giovanni Dietler, Serguei Sekatski, Mélanie Favre
2008
Conference Papers

Abstract

Using single-molecule force-clamp spectroscopy, where the distance between the AFM tip and the sample surface is fixed and a few parallel avidin-biotin complexes are kept stretched by a certain force, we were able to observe the formation of single avidin-biotin bonds. Perspectives to use such all approach to study association reactions at single-molecule level in the conditions resembling those characteristic for some processes in vivo (e.g. virus-cell membrane attachment) are briefly discussed. (C) 2008 Elsevier B.V. All rights reserved.

Official source

https://infoscience.epfl.ch/entities/publication/7b9502cc-e453-47a4-9b35-1f73ddaf4234

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Single-molecule avidin-biotin association reaction studied by force-clamp spectroscopy

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