Publication

Fuorescence lifetime micro-endoscopy (FLIMME) via single multimode fiber: a high-resolution tool for identifying head and neck cancer margins

2026

Abstract

We present a minimally invasive endomicroscope that performs frequency-domain fluorescence lifetime imaging (FD-FLIM) through a single multimode fiber (MMF), enabling quantitative metabolic contrast at microscopic resolution. The system, termed FLIMME, uses amplitude-modulated 405 nm excitation and lock-in detection to map endogenous fluorophores (NADH, FAD) with temporal accuracy of ±0.03 ns and lifetime resolution down to 0.5 ns. Validation with reference dyes shows excellent agreement with a commercial time-domain FLIM microscope. Ex vivo murine submandibular gland imaging demonstrates label-free visualization of tissue microstructure and metabolic heterogeneity. FLIMME bridges advanced photonics and translational oncology. Its ultrathin, needle-like design allows insertion into surgical sites for potential real-time metabolic imaging of tumor margins.

Official source

https://infoscience.epfl.ch/entities/publication/7ab23ba0-7fb0-47c3-94a1-e20cd75744fb

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2026

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/7ab23ba0-7fb0-47c3-94a1-e20cd75744fb

About this result

Related concepts (30)

Super-resolution microscopy

Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy as well as those that use the Pendry Superlens and near field scanning optical microscopy) or on the far-field.

Head and neck cancer

Head and neck cancer develops from tissues in the lip and oral cavity (mouth), larynx (throat), salivary glands, nose, sinuses, or skin of the face. The most common types of head and neck cancer occur in the lips, mouth, and larynx. Symptoms predominantly include a sore that does not heal or a change in the voice. In those with advanced disease, there may be unusual bleeding, facial pain, numbness or swelling, and visible lumps on the outside of the neck or oral cavity.

Resection margin

A resection margin or surgical margin is the margin of apparently non-tumorous tissue around a tumor that has been surgically removed, called "resected", in surgical oncology. The resection is an attempt to remove a cancer tumor so that no portion of the malignant growth extends past the edges or margin of the removed tumor and surrounding tissue. These are retained after the surgery and examined microscopically by a pathologist to see if the margin is indeed free from tumor cells (called "negative").

Related publications (2)

Fuorescence lifetime micro-endoscopy (FLIMME) via single multimode fiber: a high-resolution tool for identifying head and neck cancer margins

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Analysis of imaging modalities for classification of tumoral vs. normal tissues using an FD-FLIM based MMF endoscopy probe

Chemically resolved imaging of biological cells and thin films by infrared scanning near-field optical microscopy