Publication

Nucleic acid sequencing by processive degradation of dye-labeled polynucleotides immobilized on filter membranes

Horst Arthur Vogel
2003
Patents

Abstract

The invention relates to a method and a device for sequencing nucleic acid mols. on a perforated membrane. The inventive method and device can be esp. used in multiplex sequencing. The method involves prepg. an oligonucleotide that is labeled to satn. with base-specific fluorescent dyes that is then immobilized on a filter. The labeled sequence is then processively degraded with an exonuclease with the released bases being detected fluorometrically. [on SciFinder (R)]

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Horst Arthur Vogel
2003
Patents

Abstract

Official source

Related concepts (32)

Fluorescent tag

In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically to aid in the detection of a biomolecule such as a protein, antibody, or amino acid. Generally, fluorescent tagging, or labeling, uses a reactive derivative of a fluorescent molecule known as a fluorophore. The fluorophore selectively binds to a specific region or functional group on the target molecule and can be attached chemically or biologically.

Invention

An invention is a unique or novel device, method, composition, idea or process. An invention may be an improvement upon a machine, product, or process for increasing efficiency or lowering cost. It may also be an entirely new concept. If an idea is unique enough either as a stand alone invention or as a significant improvement over the work of others, it can be patented. A patent, if granted, gives the inventor a proprietary interest in the patent over a specific period of time, which can be licensed for financial gain.

Fluorophore

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions).

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Nucleic acid sequencing by processive degradation of dye-labeled polynucleotides immobilized on filter membranes

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