Publication

Investigation of nanolocal fluorescence resonance energy transfer for scanning probe microscopy

Majed Chergui, Giovanni Dietler
1999
Journal Articles

Abstract

Fluorescence resonance energy transfer (FRET) was obsd. between donor dye mols. deposited onto the sample surface and acceptor dye mols. deposited onto the scanning near-field optical microscope (SNOM) or at. force microscope tip. FRET was obsd. only when the tip acquired a contact with the sample and took place in a region of few tens of square nanometers in size when thousands (hundreds) of mols. are involved. In view of the obtained results, the perspectives for the construction of a 1-atom FRET SNOM are described. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/7496169e-2295-45cd-88d5-1b71afe16b4c

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Majed Chergui, Giovanni Dietler
1999
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/7496169e-2295-45cd-88d5-1b71afe16b4c

About this result

Related concepts (23)

Scanning probe microscopy

Scanning probe microscopy (SPM) is a branch of microscopy that forms images of surfaces using a physical probe that scans the specimen. SPM was founded in 1981, with the invention of the scanning tunneling microscope, an instrument for imaging surfaces at the atomic level. The first successful scanning tunneling microscope experiment was done by Gerd Binnig and Heinrich Rohrer. The key to their success was using a feedback loop to regulate gap distance between the sample and the probe.

Near-field scanning optical microscope

Near-field scanning optical microscopy (NSOM) or scanning near-field optical microscopy (SNOM) is a microscopy technique for nanostructure investigation that breaks the far field resolution limit by exploiting the properties of evanescent waves. In SNOM, the excitation laser light is focused through an aperture with a diameter smaller than the excitation wavelength, resulting in an evanescent field (or near-field) on the far side of the aperture.

Optical microscope

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope.

Related publications (32)

Investigation of nanolocal fluorescence resonance energy transfer for scanning probe microscopy

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