Publication

A first-in-class multiplexed high-dimensional mass cytometry platform to determine immune effector-tumor binding and cytotoxicity in antibody and cellular immunotherapy studies

2025

Abstract

Antibody- and cell-based immunotherapies rely on the capacity of immune effectors to recognize and eliminate tumor cells. However, current in vitro cytotoxicity assays offer limited resolution, throughput, and mechanistic insight into which immune populations mediate killing. To address this gap, we developed a first-in-class, multiplexed mass cytometry platform that quantifies both effector-tumor binding and functional cytotoxicity at single-cell resolution across diverse immune conditions and therapeutic formats.

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2025

Abstract

Official source

Related concepts (30)

Immune system

The immune system is a network of biological processes that protects an organism from diseases. It detects and responds to a wide variety of pathogens, from viruses to parasitic worms, as well as cancer cells and objects such as wood splinters, distinguishing them from the organism's own healthy tissue. Many species have two major subsystems of the immune system. The innate immune system provides a preconfigured response to broad groups of situations and stimuli.

Natural killer cell

Natural killer cells, also known as NK cells or large granular lymphocytes (LGL), are a type of cytotoxic lymphocyte critical to the innate immune system that belong to the rapidly expanding family of known innate lymphoid cells (ILC) and represent 5–20% of all circulating lymphocytes in humans. The role of NK cells is analogous to that of cytotoxic T cells in the vertebrate adaptive immune response. NK cells provide rapid responses to virus-infected cell and other intracellular pathogens acting at around 3 days after infection, and respond to tumor formation.

Immunotherapy

Immunotherapy or biological therapy is the treatment of disease by activating or suppressing the immune system. Immunotherapies designed to elicit or amplify an immune response are classified as activation immunotherapies, while immunotherapies that reduce or suppress are classified as suppression immunotherapies. Immunotherapy is under preliminary research for its potential to treat various forms of cancer. Cell-based immunotherapies are effective for some cancers.

Related publications (9)

A first-in-class multiplexed high-dimensional mass cytometry platform to determine immune effector-tumor binding and cytotoxicity in antibody and cellular immunotherapy studies

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