Publication

Measurement of cross-relaxation between amide protons in 15N-enriched proteins with suppression of spin diffusion

Geoffrey Bodenhausen
1996
Journal Articles

Abstract

A variant of two-dimensional nuclear Overhauser effect spectroscopy (NOESY) is described which allows one to observe cross-relaxation pathways between protons that have heteronuclear scalar couplings to nitrogen-15 or carbon-13 nuclei. In 15N-enriched proteins, it is possible to focus attention on Overhauser effects between amide protons. All Overhauser effects between amide and other protons are eliminated by inserting a bilinear rotation decoupling (BIRD) sequence in the middle of the relaxation interval. The mechanism of the suppression of cross relaxation is analogous to expts. involving selective inversion of the longitudinal magnetization of protons under investigation by quenching undesirable indirect external trouble in nuclear Overhauser effect spectroscopy (QUIET-NOESY). The new method is therefore called QUIET-BIRD-NOESY. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/7296d70e-9157-4cea-98ec-234a8387b00b

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Geoffrey Bodenhausen
1996
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/7296d70e-9157-4cea-98ec-234a8387b00b

About this result

Related concepts (21)

Nuclear Overhauser effect

The nuclear Overhauser effect (NOE) is the transfer of nuclear spin polarization from one population of spin-active nuclei (e.g. 1H, 13C, 15N etc.) to another via cross-relaxation. A phenomenological definition of the NOE in nuclear magnetic resonance spectroscopy (NMR) is the change in the integrated intensity (positive or negative) of one NMR resonance that occurs when another is saturated by irradiation with an RF field. The change in resonance intensity of a nucleus is a consequence of the nucleus being close in space to those directly affected by the RF perturbation.

Two-dimensional nuclear magnetic resonance spectroscopy

Two-dimensional nuclear magnetic resonance spectroscopy (2D NMR) is a set of nuclear magnetic resonance spectroscopy (NMR) methods which give data plotted in a space defined by two frequency axes rather than one. Types of 2D NMR include correlation spectroscopy (COSY), J-spectroscopy, exchange spectroscopy (EXSY), and nuclear Overhauser effect spectroscopy (NOESY). Two-dimensional NMR spectra provide more information about a molecule than one-dimensional NMR spectra and are especially useful in determining the structure of a molecule, particularly for molecules that are too complicated to work with using one-dimensional NMR.

Nuclear magnetic resonance spectroscopy

Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique to observe local magnetic fields around atomic nuclei. This spectroscopy is based on the measurement of absorption of electromagnetic radiations in the radio frequency region from roughly 4 to 900 MHz. Absorption of radio waves in the presence of magnetic field is accompanied by a special type of nuclear transition, and for this reason, such type of spectroscopy is known as Nuclear Magnetic Resonance Spectroscopy.

Related publications (23)

Measurement of cross-relaxation between amide protons in 15N-enriched proteins with suppression of spin diffusion

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