Publication

Mesoscopic light-sheet fluorescence imaging for drug delivery and target engagement assessment

2026

Abstract

Fluorescence lifetime imaging (FLIM) offers a powerful approach for assessing drug delivery and target engagement in targeted therapies. However, monitoring drug response in 3D at the mesoscopic scale has been largely constrained by tissue scattering and prolonged acquisition times. In this study, we present the application of our near-infrared to shortwave-infrared (NIR-SWIR) light-sheet mesoscope for rapid 3D Förster Resonance Energy Transfer (FRET) mapping in HER2-positive tumor spheroid models.

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2026

Abstract

Official source

Related concepts (17)

Infrared

Infrared (IR; sometimes called infrared light) is electromagnetic radiation (EMR) with wavelengths longer than those of visible light and shorter than radio waves. It is therefore invisible to the human eye. IR is generally understood to encompass wavelengths from around 1 millimeter (300 GHz) to the nominal red edge of the visible spectrum, around 700 nanometers (430 THz). IR is commonly divided between longer-wavelength thermal infrared that is emitted from terrestrial sources and shorter-wavelength near-infrared that is part of the solar spectrum.

Raman scattering

Raman scattering or the Raman effect (ˈrɑːmən) is the inelastic scattering of photons by matter, meaning that there is both an exchange of energy and a change in the light's direction. Typically this effect involves vibrational energy being gained by a molecule as incident photons from a visible laser are shifted to lower energy. This is called normal Stokes Raman scattering. The effect is exploited by chemists and physicists to gain information about materials for a variety of purposes by performing various forms of Raman spectroscopy.

Near-infrared spectroscopy

Near-infrared spectroscopy (NIRS) is a spectroscopic method that uses the near-infrared region of the electromagnetic spectrum (from 780 nm to 2500 nm). Typical applications include medical and physiological diagnostics and research including blood sugar, pulse oximetry, functional neuroimaging, sports medicine, elite sports training, ergonomics, rehabilitation, neonatal research, brain computer interface, urology (bladder contraction), and neurology (neurovascular coupling).

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Mesoscopic light-sheet fluorescence imaging for drug delivery and target engagement assessment

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