Publication

Detection of biomolecules using polymer containing vesicles and functionalized sensors

Horst Arthur Vogel
2000
Patents

Abstract

The invention relates to a functionalized, polymer-reinforced (sterically stabilized) vesicle which comprises a biol., biochem. or synthetic identifying element for identifying and binding a ligand. In addn., the inventive vesicle optionally contains labels which serve as signal-generating constituents in a bioanal. detection method. The invention also relates to a method for producing said vesicle and to the use thereof in detection methods. Thus, self assembled monolayers (SAMs) were produced on the surface of a surface plasmon resonance (SPR) chip, the SAMs were treated with EDC and streptavidin, and functionalized with biotinylated a-Bungarotoxin. Lipid vesicles were produced with DOPC, cholesterol and DOPG, that were stabilized with PEG; nicotinic acetylcholine receptors incorporated. A method is given for the evaluation signals that correspond to non-specific binding. Labels for other detection methods, e.g. ESR, NMR, immunoassay can be added. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/70c51a4c-9ef2-402d-b7b6-087355e0f725

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Horst Arthur Vogel
2000
Patents

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/70c51a4c-9ef2-402d-b7b6-087355e0f725

About this result

Related concepts (28)

Surface plasmon

Surface plasmons (SPs) are coherent delocalized electron oscillations that exist at the interface between any two materials where the real part of the dielectric function changes sign across the interface (e.g. a metal-dielectric interface, such as a metal sheet in air). SPs have lower energy than bulk (or volume) plasmons which quantise the longitudinal electron oscillations about positive ion cores within the bulk of an electron gas (or plasma). The charge motion in a surface plasmon always creates electromagnetic fields outside (as well as inside) the metal.

Surface plasmon resonance

Surface plasmon resonance (SPR) is a phenomenon that occurs where electrons in a thin metal sheet become excited by light that is directed to the sheet with a particular angle of incidence, and then travel parallel to the sheet. Assuming a constant light source wavelength and that the metal sheet is thin, the angle of incidence that triggers SPR is related to the refractive index of the material and even a small change in the refractive index will cause SPR to not be observed.

Localized surface plasmon

A localized surface plasmon (LSP) is the result of the confinement of a surface plasmon in a nanoparticle of size comparable to or smaller than the wavelength of light used to excite the plasmon. When a small spherical metallic nanoparticle is irradiated by light, the oscillating electric field causes the conduction electrons to oscillate coherently. When the electron cloud is displaced relative to its original position, a restoring force arises from Coulombic attraction between electrons and nuclei.

Related publications (16)

Detection of biomolecules using polymer containing vesicles and functionalized sensors

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