Publication

FABRICATION OF NANOPORES IN SIN MEMBRANES USING FIB

Horst Arthur Vogel, Christian Santschi
2006
Reports

Abstract

Pressing adhering live cells onto the nanostructured SiN substrate and subsequent removal, planar cell membrane sheets (CMSs) were transferred in a well-defined orientation onto the SiN support. Using perforated thin SiN membranes both, extracellular and intracellular surfaces of CMSs become simultaneously accessible by targeting membrane constituents side-specifically with fluorescent markers. The perforation of the membranes has been performed using FIB technique.

Official source

https://infoscience.epfl.ch/entities/publication/6e75ea2f-1343-4121-b270-a0de2a656b99

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Horst Arthur Vogel, Christian Santschi
2006
Reports

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/6e75ea2f-1343-4121-b270-a0de2a656b99

About this result

Related concepts (18)

Fluorescent tag

In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically to aid in the detection of a biomolecule such as a protein, antibody, or amino acid. Generally, fluorescent tagging, or labeling, uses a reactive derivative of a fluorescent molecule known as a fluorophore. The fluorophore selectively binds to a specific region or functional group on the target molecule and can be attached chemically or biologically.

Cell surface receptor

Cell surface receptors (membrane receptors, transmembrane receptors) are receptors that are embedded in the plasma membrane of cells. They act in cell signaling by receiving (binding to) extracellular molecules. They are specialized integral membrane proteins that allow communication between the cell and the extracellular space. The extracellular molecules may be hormones, neurotransmitters, cytokines, growth factors, cell adhesion molecules, or nutrients; they react with the receptor to induce changes in the metabolism and activity of a cell.

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The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP. However, GFPs have been found in other organisms including corals, sea anemones, zoanithids, copepods and lancelets. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm.

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