Publication

Telomerase: biochemical considerations for enzyme and substrate

2002
Journal Articles

Abstract

Telomerase extends chromosome ends by iterative reverse transcription of its RNA template. Following the addition of each telomeric repeat, the RNA template and the telomeric substrate reset their relative position in the active site provided by the telomerase reverse transcriptase (TERT). This step might require the formation of guanine-rich secondary structures in the nascent product. Results from numerous studies begin to delineate TERT sub-domains that orchestrate these events and support the model of cooperative action between distinct active sites within telomerase multimers. Natural telomere substrates are protein-DNA complexes that show an asymmetry between the two ends of a chromosome, possibly reflecting their differential mode of replication.

Official source

https://infoscience.epfl.ch/entities/publication/6ac57aea-46b9-40ca-a37d-c7e3213b9c06

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2002
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/6ac57aea-46b9-40ca-a37d-c7e3213b9c06

About this result

Related concepts (32)

Chromosome

A chromosome is a long DNA molecule with part or all of the genetic material of an organism. In most chromosomes the very long thin DNA fibers are coated with packaging proteins; in eukaryotic cells the most important of these proteins are the histones. These proteins, aided by chaperone proteins, bind to and condense the DNA molecule to maintain its integrity. These chromosomes display a complex three-dimensional structure, which plays a significant role in transcriptional regulation.

Telomerase

Telomerase, also called terminal transferase, is a ribonucleoprotein that adds a species-dependent telomere repeat sequence to the 3' end of telomeres. A telomere is a region of repetitive sequences at each end of the chromosomes of most eukaryotes. Telomeres protect the end of the chromosome from DNA damage or from fusion with neighbouring chromosomes. The fruit fly Drosophila melanogaster lacks telomerase, but instead uses retrotransposons to maintain telomeres. Telomerase is a reverse transcriptase enzyme that carries its own RNA molecule (e.

Telomerase reverse transcriptase

Telomerase reverse transcriptase (abbreviated to TERT, or hTERT in humans) is a catalytic subunit of the enzyme telomerase, which, together with the telomerase RNA component (TERC), comprises the most important unit of the telomerase complex. Telomerases are part of a distinct subgroup of RNA-dependent polymerases. Telomerase lengthens telomeres in DNA strands, thereby allowing senescent cells that would otherwise become postmitotic and undergo apoptosis to exceed the Hayflick limit and become potentially immortal, as is often the case with cancerous cells.

Related publications (10)

Telomerase: biochemical considerations for enzyme and substrate

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FluoTag-EMSA: a fast and accessible quantitative method to assess RNA-binding specificity using 3′-tagged hybrid duplexes

TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo

An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase