Publication

Separation of antibiotic-treated mycobacterial subpopulations using multiple-frequency dielectrophoresis

Philippe Renaud
2009
Conference Papers

Abstract

Exposure of a bacterial culture to an antibiotic or other lethal stress typically results in the generation of sub-populations of live cells ("persisters") and dead cells, and, in some cases, viable but non-culturable cells. Studies aimed at understanding the mechanisms of antibiotic killing and bacterial persistence will require the purification and analysis of each of these sub-populations in isolation. This paper reports a method based on continuous-flow dielectrophoresis to separate live and dead cells from antibiotic-treated bacterial cultures. Our experiments were carried out on Mycobacterium smegmatis, a model organism that is closely related to the etiological agent of human tuberculosis.

Official source

https://infoscience.epfl.ch/entities/publication/67871f4b-5d67-438f-bdce-589c531c9934

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Philippe Renaud
2009
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/67871f4b-5d67-438f-bdce-589c531c9934

About this result

Related concepts (32)

Antibiotic

An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting bacterial infections, and antibiotic medications are widely used in the treatment and prevention of such infections. They may either kill or inhibit the growth of bacteria. A limited number of antibiotics also possess antiprotozoal activity. Antibiotics are not effective against viruses such as the common cold or influenza; drugs which inhibit growth of viruses are termed antiviral drugs or antivirals rather than antibiotics.

Antibiotic sensitivity testing

Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics. It is used because bacteria may have resistance to some antibiotics. Sensitivity testing results can allow a clinician to change the choice of antibiotics from empiric therapy, which is when an antibiotic is selected based on clinical suspicion about the site of an infection and common causative bacteria, to directed therapy, in which the choice of antibiotic is based on knowledge of the organism and its sensitivities.

Antibiotic use in livestock

Antibiotic use in livestock is the use of antibiotics for any purpose in the husbandry of livestock, which includes treatment when ill (therapeutic), treatment of a group of animals when at least one is diagnosed with clinical infection (metaphylaxis), and preventative treatment (prophylaxis). Antibiotics are an important tool to treat animal as well as human disease, safeguard animal health and welfare, and support food safety. However, used irresponsibly, this may lead to antibiotic resistance which may impact human, animal and environmental health.

Related publications (13)

Separation of antibiotic-treated mycobacterial subpopulations using multiple-frequency dielectrophoresis

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