Publication

Optimisation of the formation and distribution of protoporphyrin IX in the urothelium: An in vitro approach

Hubert van den Bergh, Norbert Lange
1999
Journal Articles

Abstract

PURPOSE: To optimize conditions for photodynamic detection (PDD) and photodynamic therapy (PDT) of bladder carcinoma, urothelial accumulation of protoporphyrin IX (PpIX) and conditions leading to cell photodestruction were studied. MATERIALS AND METHODS: Porcine and human bladder mucosae were superfused with derivatives of 5-aminolevulinic acid (ALA). PpIX accumulation and distribution across the mucosa was studied by microspectrofluorometry. Cell viability and structural integrity were assessed by using vital dyes and microscopy. RESULTS: ALA esters, especially hexyl-ALA, accelerated and regularized urothelial PpIX accumulation and allowed for necrosis upon illumination. CONCLUSIONS: hexyl-ALA used at micromolar concentrations is the most efficient PpIX precursor for PDD and PDT.

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https://infoscience.epfl.ch/entities/publication/664b3e2d-8aed-4cc6-a7f3-04fade6306b3

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Hubert van den Bergh, Norbert Lange
1999
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/664b3e2d-8aed-4cc6-a7f3-04fade6306b3

About this result

Related concepts (32)

Photodynamic therapy

Photodynamic therapy (PDT) is a form of phototherapy involving light and a photosensitizing chemical substance, used in conjunction with molecular oxygen to elicit cell death (phototoxicity). PDT is popularly used in treating acne. It is used clinically to treat a wide range of medical conditions, including wet age-related macular degeneration, psoriasis, atherosclerosis and has shown some efficacy in anti-viral treatments, including herpes. It also treats malignant cancers including head and neck, lung, bladder and particular skin.

Bladder cancer

Bladder cancer is any of several types of cancer arising from the tissues of the urinary bladder. Symptoms include blood in the urine, pain with urination, and low back pain. It is caused when epithelial cells that line the bladder become malignant. Risk factors for bladder cancer include smoking, family history, prior radiation therapy, frequent bladder infections, and exposure to certain chemicals. The most common type is transitional cell carcinoma. Other types include squamous cell carcinoma and adenocarcinoma.

Carcinoma

Carcinoma is a malignancy that develops from epithelial cells. Specifically, a carcinoma is a cancer that begins in a tissue that lines the inner or outer surfaces of the body, and that arises from cells originating in the endodermal, mesodermal or ectodermal germ layer during embryogenesis. Carcinomas occur when the DNA of a cell is damaged or altered and the cell begins to grow uncontrollably and become malignant. It is from the καρκίνωμα (itself derived from karkinos meaning crab).Oxford English Dictionary, 3rd edition, s.

Related publications (2)

Optimisation of the formation and distribution of protoporphyrin IX in the urothelium: An in vitro approach

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Detection of early bladder carcinoma by fluorescence cystoscopy with Hexvix(R): Improvement of the specificity by microcystoscopy

Comparison of aminolevulinic acid and hexylester aminolevulinate induced protoporphyrin IX distribution in human bladder cancer