Publication

Development of a cGMP recorder with chemical labeling

Kai Johnsson
2026

Abstract

Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger, that regulates the cardiovascular system, brain functions and is involved in many pathological processes. To visualize cellular dynamics of cGMP levels, fluorescent protein-based sensors have been developed. However, these fluorescent sensors are not well suited to monitor changes in cGMP levels in large cell populations or in vivo. Here, we introduce a molecular recorder to transform transient changes in cGMP levels into permanent fluorescent marks for later analysis. Our cGMP recorder is based on the split-HaloTag system, which is intramolecularly fused to PKG and a cGMP-dependent PKG binder. Only upon cGMP binding, functional HaloTag is reconstituted and can be labeled with fluorescent substrates. The recorder has a large dynamic range (up to 11-fold change) and good cGMP-specificity. Thus, our cGMP recorder should help to link changes in cGMP levels to biological phenotypes.

Official source

https://infoscience.epfl.ch/entities/publication/626c70d5-1e4e-4b0e-9c0f-506c9a053174

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Kai Johnsson
2026

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/626c70d5-1e4e-4b0e-9c0f-506c9a053174

About this result

Related concepts (32)

Green fluorescent protein

The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP. However, GFPs have been found in other organisms including corals, sea anemones, zoanithids, copepods and lancelets. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm.

Guanylate cyclase

Guanylate cyclase (EC 4.6.1.2, also known as guanyl cyclase, guanylyl cyclase, or GC; systematic name GTP diphosphate-lyase (cyclizing; 3′,5′-cyclic-GMP-forming)) is a lyase enzyme that converts guanosine triphosphate (GTP) to cyclic guanosine monophosphate (cGMP) and pyrophosphate: GTP = 3′,5′-cyclic GMP + diphosphate It is often part of the G protein signaling cascade that is activated by low intracellular calcium levels and inhibited by high intracellular calcium levels.

Cyclic guanosine monophosphate

Cyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from guanosine triphosphate (GTP). cGMP acts as a second messenger much like cyclic AMP. Its most likely mechanism of action is activation of intracellular protein kinases in response to the binding of membrane-impermeable peptide hormones to the external cell surface. Through protein kinases activation, cGMP can relax smooth muscle. cGMP concentration in urine can be measured for kidney function and diabetes detection.