Publication

Aluminium Foil as Single-Use Substrate for MALDI-MS Fingerprinting of Different Melanoma Cell Lines

Hubert Girault, Horst Pick
2016
Journal Articles

Abstract

Herein, we present the intact cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the fingerprinting of human melanoma cancer cell lines grown on aluminium foil. To perform the MALDI-MS assay, melanoma cells were cultured on a flat and thin foil, which was directly transferred to the target plate of MALDI-MS for analysis. The influence of a wide range of cell fixation protocols (i.e. formalin-based and alcohol-based methods) and MALDI matrices on the obtained characteristic spectra was investigated. For the optimization of the MALDI-MS protocol, the MS fingerprints of the melanoma WM-239 cell line with and without an overexpressed enhanced green fluorescent protein were employed. The fingerprints obtained from WM-239 cells grown on aluminum foil were compared with intact cell MALDI of cell pellet and presented higher sensitivity in high m/z range. The optimized protocol was subsequently applied to characterise melanoma cell lines derived from different cancer stages and allowed the identification of unique MS signals that can be used for the differentiation between the studied cell lines (i.e. molecular weight equal to 10.0 kDa and 26.1 kDa).

Official source

https://infoscience.epfl.ch/entities/publication/5d071646-6dec-406f-acb6-f309efa61804

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Hubert Girault, Horst Pick
2016
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/5d071646-6dec-406f-acb6-f309efa61804

About this result

Related concepts (32)

Matrix-assisted laser desorption/ionization

In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy-absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods.

Melanoma

Melanoma, also redundantly known as malignant melanoma, is a type of cancer that develops from the pigment-producing cells known as melanocytes. Melanomas typically occur in the skin, but may rarely occur in the mouth, intestines, or eye (uveal melanoma). In women, they most commonly occur on the legs, while in men, they most commonly occur on the back. About 25% of melanomas develop from moles. Changes in a mole that can indicate melanoma include an increase in size, irregular edges, change in color, itchiness, or skin breakdown.

Cancer staging

Cancer staging is the process of determining the extent to which a cancer has grown and spread. A number from I to IV is assigned, with I being an isolated cancer and IV being a cancer that has metastasized and spread from its origin. The stage generally takes into account the size of a tumor, whether it has invaded adjacent organs, how many regional (nearby) lymph nodes it has spread to (if any), and whether it has appeared in more distant locations (metastasized).

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Aluminium Foil as Single-Use Substrate for MALDI-MS Fingerprinting of Different Melanoma Cell Lines

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