Publication

Orbitally shaken bioreactors for mammalian cell culture : engineering characterization and bioprocess scale-up

2009
Theses

Abstract

Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel, cost-effective mammalian cell culture bioreactor systems for the production of biologicals requiring mammalian protein processing. Recently, orbitally shaken bioreactors at scales from a few mLs to 2'000-L have been explored for the cultivation of mammalian cells and are considered to be attractive alternatives to conventional stirred-tank bioreactors because of increased flexibility and reduced costs. Although the choice of geometry of these shaken containers is diverse with the availability of conical (i.e. shake flask), square-shaped, or cylindrical containers, the latter are preferred because more regular and predictable flow patterns are generated at all volumetric scales. The main challenge facing the scale-up of the shaken container is to maintain a moderate oxygen transfer rate (OTR) to meet the oxygen requirement of cultured cells. The volumetric mass transfer coefficient (KLa) of shaken bioreactors with working volumes from a few mLs to 1'000-L were determined, and operating parameters having an impact on the oxygen transfer were investigated. KLa values between 10-30 h-1 were typically achieved in small-scale (

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2009
Theses

Abstract

Official source

Related concepts (32)

Volume

Volume is a measure of three-dimensional space. It is often quantified numerically using SI derived units (such as the cubic metre and litre) or by various imperial or US customary units (such as the gallon, quart, cubic inch). The definition of length (cubed) is interrelated with volume. The volume of a container is generally understood to be the capacity of the container; i.e., the amount of fluid (gas or liquid) that the container could hold, rather than the amount of space the container itself displaces.

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

Protein production

Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. This includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations.

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Orbitally shaken bioreactors for mammalian cell culture : engineering characterization and bioprocess scale-up

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