Publication

Synthesis of selectively 15N-labeled 2'-O-{[(triisopropylsilyl)oxy]methyl}(=tom)-protected ribonucleoside phosphoramidites and their incorporation into a bistable 32mer RNA sequence

2003
Journal Articles

Abstract

We present optimized reaction conditions for the conversion of 2'-O-{[(triisopropylsilyl)oxy]methyl}(tom) protected uridine and adenosine nucleosides into the corresponding protected (3-15N)-labeled uridine and cytidine and (1-15N)-labeled adenosine and guanosine nucleosides. On a DNA synthesizer, the resulting 15N-labeled 2'-O-tom-protected phosphoramidite building blocks were efficiently incorporated into five selected positions of a hairpin bi-stable 32mer RNA sequence. By 2D-HSQC and HNN-COSY expts. in H2O/D2O 9:1, the 15N-signals of all base-paired 15N-labeled nucleotides could be identified and attributed to one of the two coexisting structures of 32mer RNA sequence. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/5a8c37de-fa9f-49d1-97e6-ea8e11a42dfd

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2003
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/5a8c37de-fa9f-49d1-97e6-ea8e11a42dfd

About this result

Related concepts (31)

Adenosine triphosphate

Adenosine triphosphate (ATP) is an organic compound that provides energy to drive and support many processes in living cells, such as muscle contraction, nerve impulse propagation, condensate dissolution, and chemical synthesis. Found in all known forms of life, ATP is often referred to as the "molecular unit of currency" of intracellular energy transfer. When consumed in metabolic processes, it converts either to adenosine diphosphate (ADP) or to adenosine monophosphate (AMP). Other processes regenerate ATP.

Isotopic labeling

Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope (an atom with a detectable variation in neutron count) through a reaction, metabolic pathway, or cell. The reactant is 'labeled' by replacing specific atoms by their isotope. The reactant is then allowed to undergo the reaction. The position of the isotopes in the products is measured to determine the sequence the isotopic atom followed in the reaction or the cell's metabolic pathway.

Oligonucleotide synthesis

Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. Whereas enzymes synthesize DNA and RNA only in a 5' to 3' direction, chemical oligonucleotide synthesis does not have this limitation, although it is most often carried out in the opposite, 3' to 5' direction.

Related publications (1)

Synthesis of selectively 15N-labeled 2'-O-{[(triisopropylsilyl)oxy]methyl}(=tom)-protected ribonucleoside phosphoramidites and their incorporation into a bistable 32mer RNA sequence

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