Publication

Integrated Nanoreactor Systems: Triggering the Release and Mixing of Compounds Inside Single Vesicles

Horst Arthur Vogel, Dimitrios Stamou, Pierre-Yves Bolinger
2004
Journal Articles

Abstract

We present a method that allows the on-demand release and mixing of zepto- to femtoliter vols. of solns. in the interior of vesicular nanoreactors. The reactors comprise a nested system of lipid vesicles, part of which release their cargo in the interior of the others during a thermotropic phase transition. The performance of individual reactors immobilized on glass is characterized using confocal microscopy and a fluorescent dye that reports diln. during the release. The results confirm the predicted temp.-induced response and reveal a release transition width of 3 DegC with a half time of .apprx.1 min. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/5a016de3-3f95-422d-8aa1-1db026dcebb7

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Horst Arthur Vogel, Dimitrios Stamou, Pierre-Yves Bolinger
2004
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/5a016de3-3f95-422d-8aa1-1db026dcebb7

About this result

Related concepts (28)

Lipid bilayer

The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around all cells. The cell membranes of almost all organisms and many viruses are made of a lipid bilayer, as are the nuclear membrane surrounding the cell nucleus, and membranes of the membrane-bound organelles in the cell. The lipid bilayer is the barrier that keeps ions, proteins and other molecules where they are needed and prevents them from diffusing into areas where they should not be.

Vesicle (biology and chemistry)

In cell biology, a vesicle is a structure within or outside a cell, consisting of liquid or cytoplasm enclosed by a lipid bilayer. Vesicles form naturally during the processes of secretion (exocytosis), uptake (endocytosis), and the transport of materials within the plasma membrane. Alternatively, they may be prepared artificially, in which case they are called liposomes (not to be confused with lysosomes). If there is only one phospholipid bilayer, the vesicles are called unilamellar liposomes; otherwise they are called multilamellar liposomes.

Endomembrane system

The endomembrane system is composed of the different membranes (endomembranes) that are suspended in the cytoplasm within a eukaryotic cell. These membranes divide the cell into functional and structural compartments, or organelles. In eukaryotes the organelles of the endomembrane system include: the nuclear membrane, the endoplasmic reticulum, the Golgi apparatus, lysosomes, vesicles, endosomes, and plasma (cell) membrane among others.

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Integrated Nanoreactor Systems: Triggering the Release and Mixing of Compounds Inside Single Vesicles

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