Publication

A calcium ion-selective electrode array for monitoring the activity of HepG2/C3As in a microchannel

Philippe Renaud
2012
Journal Articles

Abstract

Variations in calcium ion (Ca2+) concentrations play a crucial role in cell-cell communication; periodic changes act as cellular signals. In this study, we developed a solid-state calcium ion-selective electrode (Ca2+ ISE) integrated in a microchannel to measure extracellular Ca2+ variations; an ISE has a high detection sensitivity (as low as 10(-9) M) and selectivity against potassium ions (K+). The sensor was used to monitor the cellular activities of HepG2/C3As cultured in a microchannel. We measured a periodically oscillating extracellular Ca2+ concentration that was superimposed on a gradual increase in the Ca2+ concentration after potassium chloride (KCl) stimulation. The oscillation period was in the range of 30-50 s, which was in good agreement with the literature.

Official source

https://infoscience.epfl.ch/entities/publication/59333efb-1355-4f6b-bb45-95d332c4bfac

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Philippe Renaud
2012
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/59333efb-1355-4f6b-bb45-95d332c4bfac

About this result

Related concepts (32)

Potassium

Potassium is the chemical element with the symbol K (from Neo-Latin kalium) and atomic number 19. It is a silvery white metal that is soft enough to easily cut with a knife. Potassium metal reacts rapidly with atmospheric oxygen to form flaky white potassium peroxide in only seconds of exposure. It was first isolated from potash, the ashes of plants, from which its name derives. In the periodic table, potassium is one of the alkali metals, all of which have a single valence electron in the outer electron shell, which is easily removed to create an ion with a positive charge (which combines with anions to form salts).

Calcium in biology

Calcium ions (Ca2+) contribute to the physiology and biochemistry of organisms' cells. They play an important role in signal transduction pathways, where they act as a second messenger, in neurotransmitter release from neurons, in contraction of all muscle cell types, and in fertilization. Many enzymes require calcium ions as a cofactor, including several of the coagulation factors. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.

Potassium chloride

Potassium chloride (KCl, or potassium salt) is a metal halide salt composed of potassium and chlorine. It is odorless and has a white or colorless vitreous crystal appearance. The solid dissolves readily in water, and its solutions have a salt-like taste. Potassium chloride can be obtained from ancient dried lake deposits. KCl is used as a fertilizer, in medicine, in scientific applications, domestic water softeners (as a substitute for sodium chloride salt), and in food processing, where it may be known as E number additive E508.

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A calcium ion-selective electrode array for monitoring the activity of HepG2/C3As in a microchannel

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