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This protocol comprises various methods to coculture organoids (particularly human small intestinal and colon organoids) with microbes, including microinjection into the lumen and periphery of 3D organoids and exposure of organoids to microbes in a 2D layer.
Bart Deplancke, Riccardo Dainese, Daniel Alpern, Mustafa Demir, Dariia Gudkova
Tamar Kohn, Xavier Fernandez Cassi