Publication

Incorporation of melittin into phosphatidylcholine bilayers. Study of binding and conformational changes

Horst Arthur Vogel
1981
Journal Articles

Abstract

CD and fluorescence were used to study the interaction of the model peptide melittin with dimyristoylphosphatidylcholine (DMPC) bilayer liposomes. Melittin binds tightly to the lipid membrane and undergoes a conformational change from a random to an .apprx.70% a-helical structure in the membrane-bound state. This binding is influenced by the phys. state of the membrane, the binding const. being higher in the fluid than in the ordered membrane phase. Tryptophan-19 of melittin which is positioned in the region of the glycerol backbone of DMPC, has a lower solvent accessibility in the membrane as compared to that in aq. soln. At temps. above the lipid phase transition the tryptophan residue is buried deeper within the membrane and is in a more hydrophobic microenvironment. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/527cf3db-83ad-45b1-b729-07ebff65f61e

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Horst Arthur Vogel
1981
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/527cf3db-83ad-45b1-b729-07ebff65f61e

About this result

Related concepts (32)

Lipid bilayer

The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around all cells. The cell membranes of almost all organisms and many viruses are made of a lipid bilayer, as are the nuclear membrane surrounding the cell nucleus, and membranes of the membrane-bound organelles in the cell. The lipid bilayer is the barrier that keeps ions, proteins and other molecules where they are needed and prevents them from diffusing into areas where they should not be.

Cell membrane

The cell membrane (also known as the plasma membrane or cytoplasmic membrane, and historically referred to as the plasmalemma) is a biological membrane that separates and protects the interior of a cell from the outside environment (the extracellular space). The cell membrane consists of a lipid bilayer, made up of two layers of phospholipids with cholesterols (a lipid component) interspersed between them, maintaining appropriate membrane fluidity at various temperatures.

Lipid bilayer phase behavior

One property of a lipid bilayer is the relative mobility (fluidity) of the individual lipid molecules and how this mobility changes with temperature. This response is known as the phase behavior of the bilayer. Broadly, at a given temperature a lipid bilayer can exist in either a liquid or a solid phase. The solid phase is commonly referred to as a “gel” phase. All lipids have a characteristic temperature at which they undergo a transition (melt) from the gel to liquid phase.

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Incorporation of melittin into phosphatidylcholine bilayers. Study of binding and conformational changes

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