Publication

Comparison of different bioreactors and media for the production of specific monoclonal IgA antibodies

Urs von Stockar, Ian William Marison
1995
Conference Papers

Abstract

An IgA producing hybridoma cell line has been grown in various bioreactor systems, in serum-contg. and serum-free media. Bioreactors and media have been compared on the basis of product quantity (total IgA) and quality (activity, monomer/dimer/polymer ratios). IgA was produced in vitro with an activity similar to ascites fluid. The serum-free media was found to be better for such purposes. Prodn. in hollow fiber reactors resulted in high concns. of product, but with a lower activity fraction, possibly due to the formation of aggregates. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/51ee9082-2abb-41c5-90d4-1cf8745d56e8

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Urs von Stockar, Ian William Marison
1995
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/51ee9082-2abb-41c5-90d4-1cf8745d56e8

About this result

Related concepts (13)

Bioreactor

A bioreactor refers to any manufactured device or system that supports a biologically active environment. In one case, a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. This process can either be aerobic or anaerobic. These bioreactors are commonly cylindrical, ranging in size from litres to cubic metres, and are often made of stainless steel. It may also refer to a device or system designed to grow cells or tissues in the context of cell culture.

Ascites

Ascites is the abnormal build-up of fluid in the abdomen. Technically, it is more than 25 ml of fluid in the peritoneal cavity, although volumes greater than one liter may occur. Symptoms may include increased abdominal size, increased weight, abdominal discomfort, and shortness of breath. Complications can include spontaneous bacterial peritonitis. In the developed world, the most common cause is liver cirrhosis. Other causes include cancer, heart failure, tuberculosis, pancreatitis, and blockage of the hepatic vein.

Antibody

An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the pathogen, called an antigen. Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (analogous to a key) on an antigen, allowing these two structures to bind together with precision.