Publication

A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System

Sebastian Maerkl, Barbora Lavickova
2019
Journal Articles

Abstract

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.

Official source

https://infoscience.epfl.ch/entities/publication/502a108b-1efd-4f61-afe1-41edb13a4e1b

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System

Chat with Graph Search

Photoelectrochemical Cell Engineering for Solar Energy Conversion

Three-dimensional nanoimaging of fuel cell catalyst layers

Decoupling the Contributions of Different Instability Mechanisms to the PEMFC Performance Decay of Non-noble Metal O2-Reduction Catalysts