Publication

Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

Enrico Tenaglia
2018
Journal Articles

Abstract

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplification in minimally buffered conditions producing, as a readout, pH shifts attaining subunits of pH.

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Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

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