Publication

Metabolic network analysis of a hybridoma cell line using mass balances and 14C-labeled glucose and glutamine

Urs von Stockar, Ian William Marison
1998
Conference Papers

Abstract

Continuous cultures of a hybridoma cell line (Zac3) were performed with 2 14C-labeled substrates: glucose and glutamine. A simplified network was used to describe cell metab. This simple network gave a good idea of the main fluxes into the cell, esp. for catabolic reactions. The use of 14C-labeled glucose and glutamine enabled the metabolic routes to be described with great accuracy, esp. CO2 prodn. rate. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/4ea5d218-bd99-46c3-bf98-5e9873021a62

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Urs von Stockar, Ian William Marison
1998
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/4ea5d218-bd99-46c3-bf98-5e9873021a62

About this result

Related concepts (32)

Metabolic pathway

In biochemistry, a metabolic pathway is a linked series of chemical reactions occurring within a cell. The reactants, products, and intermediates of an enzymatic reaction are known as metabolites, which are modified by a sequence of chemical reactions catalyzed by enzymes. In most cases of a metabolic pathway, the product of one enzyme acts as the substrate for the next. However, side products are considered waste and removed from the cell. These enzymes often require dietary minerals, vitamins, and other cofactors to function.

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

Metabolism

Metabolism (məˈtæbəlɪzəm, from μεταβολή metabolē, "change") is the set of life-sustaining chemical reactions in organisms. The three main functions of metabolism are: the conversion of the energy in food to energy available to run cellular processes; the conversion of food to building blocks for proteins, lipids, nucleic acids, and some carbohydrates; and the elimination of metabolic wastes. These enzyme-catalyzed reactions allow organisms to grow and reproduce, maintain their structures, and respond to their environments.

Related publications (14)

Metabolic network analysis of a hybridoma cell line using mass balances and 14C-labeled glucose and glutamine

Chat with Graph Search

Asymmetric mitochondrial trafficking balances perinuclear biogenesis to maintain network morphology

Studying cancer cells phenotypes integrating omics data in human reduced genome-scale metabolic models.

iSCHRUNK – In Silico Approach to Characterization and Reduction of Uncertainty in the Kinetic Models of Genome-scale Metabolic Networks