Publication

Characterization of the promastigote surface protease of Leishmania as a membrane-bound zinc endopeptidase

Horst Arthur Vogel
1989
Journal Articles

Abstract

The effects of a variety of inhibitors suggested that the promastigote surface protease (PSP) of Leishmania might be a Zn metalloprotease. To investigate this possibility, at. emission and absorption spectroscopic analyses were conducted which showed that PSP contains 1 atom of Zn per 63-kilodalton (kDa) monomer. Further studies showed that the enzyme could be biosynthetically labeled with 65ZnCl2. The comparison of the amino acid sequence of L. major PSP with 9 other Zn metalloproteases revealed significant similarity in the area of their Zn-binding sites. These data showed clearly that the promastigote surface protease of Leishmania is a Zn metalloprotease. Secondary structure anal. by CD spectroscopy indicated that PSP contained >40% b-strand and

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https://infoscience.epfl.ch/entities/publication/4aff0f6f-d064-4a05-8db7-9188533946b3

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Horst Arthur Vogel
1989
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/4aff0f6f-d064-4a05-8db7-9188533946b3

About this result

Related concepts (32)

Spectroscopy

Spectroscopy is the field of study that measures and interprets the electromagnetic spectra that result from the interaction between electromagnetic radiation and matter as a function of the wavelength or frequency of the radiation. Matter waves and acoustic waves can also be considered forms of radiative energy, and recently gravitational waves have been associated with a spectral signature in the context of the Laser Interferometer Gravitational-Wave Observatory (LIGO).

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Characterization of the promastigote surface protease of Leishmania as a membrane-bound zinc endopeptidase

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