Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy

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High-throughput cryogenic spectroscopy for glycan analysis

Thomas Rizzo, Ahmed Ben Faleh, Valeriu Scutelnic, Stephan Warnke

A method for analyzing a plurality of molecules with cryogenic vibrational spectroscopy including the steps of providing a packet of molecules in a ionized form, injecting the packet into an ion mobility section, spatially separating the ions of the packet ...

2019

Separation and Identification of Glycan Anomers Using Ultrahigh-Resolution Ion Mobility Spectrometry and Cryogenic Ion Spectroscopy

Thomas Rizzo, Ahmed Ben Faleh, Valeriu Scutelnic, Stephan Warnke

The analysis of carbohydrates, or glycans, is challenging for established structure-sensitive gas-phase methods. The multitude of possible stereo-, regio- and structural isomers make them substantially more complex to ana-lyze than DNA or proteins, and no ...

2019

Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers

Thomas Rizzo, Valeriu Scutelnic

We combine conformer-selective, cryogenic infrared spectroscopy, quantum mechanical computations, and 18O substitution at the reducing end to determine the structural preferences of protonated glucosamine in the gas phase. Cryogenic infrared-infrared (IR-I ...

2019

Cryogenic Vibrational Spectroscopy Provides Unique Fingerprints for Glycan Identification

Thomas Rizzo, Michael Zachary Kamrath, Chiara Masellis, Neelam Khanal

The structural characterization of glycans by mass spectrometry is particularly challenging. This is because of the high degree of isomerism in which glycans of the same mass can differ in their stereochemistry, attachment points, and degree of branching. ...

2017

Cryogenic Ion Spectroscopy of Peptides and Glycans

Chiara Masellis

Understanding the structure of biomolecules, which are directly related to physiological processes that take place in the human body, has always generated a large interest in the scientific community. In this thesis we focus our attention on two of these c ...

EPFL2017

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Marc Moniatte, Valentin Juvet, Dominique Terry Monteil Jr

Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...

American Chemical Society2016

Folding and Endocytosis of Anthrax Toxin Receptors

Bacillus anthracis is the causative agent of the infectious disease anthrax. The disease is caused after taking up the spores by either inhalation, ingestion or through skin lesion. They then germinate inside the body and start secreting the tripartite ant ...

EPFL2016

Molecular Mechanism of Flocculation Self-Recognition in Yeast and Its Role in Mating and Survival

Jens Nielsen, Sandor Kasas, Livan Bladimir Alonso Sarduy

We studied the flocculation mechanism at the molecular level by determining the atomic structures of N-Flo1p and N-Lg-Flo1p in complex with their ligands. We show that they have similar ligand binding mechanisms but distinct carbohydrate specificities and ...

American Society for Microbiology2015

N-linked sugar-regulated protein folding and quality control in the ER

Maurizio Molinari

Asparagine-linked glycans (N-glycans) are displayed on the majority of proteins synthesized in the endoplasmic reticulum (ER). Removal of the outermost glucose residue recruits the lectin chaperone malectin possibly involved in a first triage of defective ...

Academic Press Ltd- Elsevier Science Ltd2015

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Zuzana Kadlecova, Sowmya Balasubramanian

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...

Elsevier2015

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