Publication

Quantitative Measurements of Dynamic Cell Morphometry and Intracellular Integral Refractive Index with Digital Holographic Microscopy

Christian Depeursinge, Tristan Colomb
2006
Conference Papers

Abstract

A digital holographic microscope, permitting to monitor cellular dynamics by measuring the cellular phase shift with a high temporal stability and a procedure allowing to calculate the refractive index and the cellular thickness are presented.

Official source

Graph Chat

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.

Christian Depeursinge, Tristan Colomb
2006
Conference Papers

Abstract

Official source

Related concepts (22)

Optical microscope

The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope.

Quantitative phase-contrast microscopy

FORCETOC Quantitative phase contrast microscopy or quantitative phase imaging are the collective names for a group of microscopy methods that quantify the phase shift that occurs when light waves pass through a more optically dense object. Translucent objects, like a living human cell, absorb and scatter small amounts of light. This makes translucent objects much easier to observe in ordinary light microscopes. Such objects do, however, induce a phase shift that can be observed using a phase contrast microscope.

Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image.

Related publications (10)

Quantitative Measurements of Dynamic Cell Morphometry and Intracellular Integral Refractive Index with Digital Holographic Microscopy

Chat with Graph Search

Leakage radiation interference microscopy

Advanced optical characterization of micro solid immersion lens

Second harmonic phase microscopy of collagen fibers