Publication

Reversible oriented immobilization of histidine-tagged proteins on gold surfaces using a chelator thioalkane

Horst Arthur Vogel
1996
Journal Articles

Abstract

The reversible oriented immobilization of proteins on solid surfaces is a prerequisite for the investigation of mol. interactions at interfaces or the construction of supramol. assemblies. We demonstrate a generally applicable method using a synthetic chelator thioalkane which can self-assemble on a gold surface via its thiol group. It exposes its nitrilotriacetic acid group which serves as a chelator for transition metal ions. Reversible binding of a Fab fragment modified with a C-terminal hexahistidine extension was monitored in situ using surface plasmon resonance. The directed immobilization of proteins on surfaces opens new ways for structural investigations of proteins and the development of biosensors. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/46aef03e-2820-4d53-8c36-4b1ea0fb04d6

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Horst Arthur Vogel
1996
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/46aef03e-2820-4d53-8c36-4b1ea0fb04d6

About this result

Related concepts (32)

Localized surface plasmon

A localized surface plasmon (LSP) is the result of the confinement of a surface plasmon in a nanoparticle of size comparable to or smaller than the wavelength of light used to excite the plasmon. When a small spherical metallic nanoparticle is irradiated by light, the oscillating electric field causes the conduction electrons to oscillate coherently. When the electron cloud is displaced relative to its original position, a restoring force arises from Coulombic attraction between electrons and nuclei.

Self-assembly

Self-assembly is a process in which a disordered system of pre-existing components forms an organized structure or pattern as a consequence of specific, local interactions among the components themselves, without external direction. When the constitutive components are molecules, the process is termed molecular self-assembly. Self-assembly can be classified as either static or dynamic. In static self-assembly, the ordered state forms as a system approaches equilibrium, reducing its free energy.

Gold

Gold is a chemical element with the symbol Au () and atomic number 79. It is a bright, slightly orange-yellow, dense, soft, malleable, and ductile metal in pure form. Chemically, gold is a transition metal and a group 11 element. It is one of the least reactive chemical elements and is solid under standard conditions. Gold often occurs in free elemental (native state), as nuggets or grains, in rocks, veins, and alluvial deposits.

Related publications (16)

Reversible oriented immobilization of histidine-tagged proteins on gold surfaces using a chelator thioalkane

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