Publication

Measurement of relaxation rates of N(H) and H(alpha) backbone protons in proteins with tailored initial conditions

Geoffrey Bodenhausen
1999
Journal Articles

Abstract

Several methods are presented for the selective determination of spin-lattice and spin-spin relaxation rates of backbone protons in labeled proteins. The relaxation rates of amide protons in (15)N labeled proteins can be measured by using two-way selective cross-polarization (SCP). The measurement of H(alpha) relaxation rates can be achieved by combining this method with homonuclear Hartmann-Hahn transfer using doubly selective irradiation. Various schemes for selective or nonselective inversion of the longitudinal proton magnetization lead to different initial recovery rates. The methods have been applied to lysine K6 in (15)N-labeled human ubiquitin and to leucine L5 in (15)N- and (13)C-labeled octapeptide YGGF*LRRI (GFL) in which the marked residues are (15)N- and (13)C-labeled. Copyright 1999 Academic Press. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/43173d23-e0b3-48e8-aa73-92ec97f20e9b

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Geoffrey Bodenhausen
1999
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/43173d23-e0b3-48e8-aa73-92ec97f20e9b

About this result

Related concepts (32)

Spin–spin relaxation

In physics, the spin–spin relaxation is the mechanism by which Mxy, the transverse component of the magnetization vector, exponentially decays towards its equilibrium value in nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI). It is characterized by the spin–spin relaxation time, known as T2, a time constant characterizing the signal decay. It is named in contrast to T1, the spin–lattice relaxation time.

Spin–lattice relaxation

During nuclear magnetic resonance observations, spin–lattice relaxation is the mechanism by which the longitudinal component of the total nuclear magnetic moment vector (parallel to the constant magnetic field) exponentially relaxes from a higher energy, non-equilibrium state to thermodynamic equilibrium with its surroundings (the "lattice"). It is characterized by the spin–lattice relaxation time, a time constant known as T1.

Nuclear Overhauser effect

The nuclear Overhauser effect (NOE) is the transfer of nuclear spin polarization from one population of spin-active nuclei (e.g. 1H, 13C, 15N etc.) to another via cross-relaxation. A phenomenological definition of the NOE in nuclear magnetic resonance spectroscopy (NMR) is the change in the integrated intensity (positive or negative) of one NMR resonance that occurs when another is saturated by irradiation with an RF field. The change in resonance intensity of a nucleus is a consequence of the nucleus being close in space to those directly affected by the RF perturbation.

Related publications (32)

Measurement of relaxation rates of N(H) and H(alpha) backbone protons in proteins with tailored initial conditions

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