Publication

A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighborhood of a consensus sequence for calcium/lipid-binding proteins

Kai Johnsson
1988
Journal Articles

Abstract

Previous models of protein p36 based on proteolytic fragments describe the tail and core as 2 noninteracting domains. However, monoclonal antibody H28 recognized a discontinuous epitope, which covers the peptide segments around serine-25 in the tail and around glutamate-65 in the core of porcine p36. Thus, the phosphorylatable tyrosine-23 is much closer to the 1st consensus sequence (residues 46-62) of Ca2+/lipid-binding proteins than previously thought. This apposition is in line with biochem. expts. indicating an influence of core ligands on tyrosine phosphorylation and an enhanced Ca2+ requirement of the modified p36 in phospholipid binding. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/4245ae29-4f8f-43cf-ac2b-96161135e080

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Kai Johnsson
1988
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/4245ae29-4f8f-43cf-ac2b-96161135e080

About this result

Related concepts (32)

Peripheral membrane protein

Peripheral membrane proteins, or extrinsic membrane proteins, are membrane proteins that adhere only temporarily to the biological membrane with which they are associated. These proteins attach to integral membrane proteins, or penetrate the peripheral regions of the lipid bilayer. The regulatory protein subunits of many ion channels and transmembrane receptors, for example, may be defined as peripheral membrane proteins.

Lipid bilayer

The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around all cells. The cell membranes of almost all organisms and many viruses are made of a lipid bilayer, as are the nuclear membrane surrounding the cell nucleus, and membranes of the membrane-bound organelles in the cell. The lipid bilayer is the barrier that keeps ions, proteins and other molecules where they are needed and prevents them from diffusing into areas where they should not be.

Tyrosine kinase

A tyrosine kinase is an enzyme that can transfer a phosphate group from ATP to the tyrosine residues of specific proteins inside a cell. It functions as an "on" or "off" switch in many cellular functions. Tyrosine kinases belong to a larger class of enzymes known as protein kinases which also attach phosphates to other amino acids such as serine and threonine. Phosphorylation of proteins by kinases is an important mechanism for communicating signals within a cell (signal transduction) and regulating cellular activity, such as cell division.

Related publications (15)

A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighborhood of a consensus sequence for calcium/lipid-binding proteins

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