Publication

Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

Christian Depeursinge
2010
Conference Papers

Abstract

Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index across a sample (e.g. cell or neuron). In short,DHM added a new dimension to optical imaging,whic h explains why it is such an excellent instrument for metrological,but also for biological applications. We believe that DHM is,b y nature,ideally suited for nonlinear microscopy. In this work,w e review the advantages of DHM for nonlinear microscopy and present its application to determination of the axial position of nonlinear nanoparticles capable of second harmonic generation.

Official source

https://infoscience.epfl.ch/entities/publication/421d66f6-a82b-43be-8ced-2aa2a3a2ced9

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Christian Depeursinge
2010
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/421d66f6-a82b-43be-8ced-2aa2a3a2ced9

About this result

Related concepts (32)

Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy. Optical microscopy and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image.

Second-harmonic generation

Second-harmonic generation (SHG, also called frequency doubling) is a nonlinear optical process in which two photons with the same frequency interact with a nonlinear material, are "combined", and generate a new photon with twice the energy of the initial photons (equivalently, twice the frequency and half the wavelength), that conserves the coherence of the excitation. It is a special case of sum-frequency generation (2 photons), and more generally of harmonic generation.

Complete measure

In mathematics, a complete measure (or, more precisely, a complete measure space) is a measure space in which every subset of every null set is measurable (having measure zero). More formally, a measure space (X, Σ, μ) is complete if and only if The need to consider questions of completeness can be illustrated by considering the problem of product spaces. Suppose that we have already constructed Lebesgue measure on the real line: denote this measure space by We now wish to construct some two-dimensional Lebesgue measure on the plane as a product measure.

Related publications (32)

Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

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