Visualizing Biochemical Activities in Living Cells through Chemistry

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The density of a protein molecule is a key property within a variety of experimental techniques. We present a computational method for determining protein mass density that explicitly incorporates hydration effects. Our approach uses molecular dynamics sim ...

Proteins are the molecular machines of life, driving essential biological processes such as enzymatic catalysis, cellular signaling, and immune responses. The ability to design entirely new proteins with desired structures and functions has immense implica ...

Apoptosis controls cell fate, ensuring tissue homeostasis and promoting disease when dysregulated. The rate-limiting step in apoptosis is mitochondrial poration by the effector B cell lymphoma 2 (BCL-2) family proteins BAK and BAX, which are activated by i ...

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins t ...

Redox homeostasis is a key factor in maintaining cellular function and health. The main determinant of the intracellular redox potential is ubiquitous glutathione (GSH) together with its oxidized dimer (GSSG). Importantly, both redox equilibrium and GSH ho ...

The phenomenon of allostery, a general property in proteins that has been heralded as "the second secret of life" remains elusive to our understanding and even more challenging to incorporate into protein design. One example of allosteric proteins with gre ...

In this paper, we quantify weak protein-protein interactions in solution using cross-interaction chromatography (CIC) and surface plasmon resonance (SPR) and demonstrate that they can be modulated by the addition of millimolar concentrations of free amino ...

In transitioning toward a sustainable economy, mycelial materials are recognized for their adaptability, biocompatibility, and eco-friendliness. This paper updates the exploration of mycelial materials, defining their scope and emphasizing the need for pre ...

Over the past two decades, protein S-acylation (often referred to as S-palmitoylation) has emerged as an important regulator of vital signalling pathways. S-Acylation is a reversible post-translational modification that involves the attachment of a fatty a ...

Proteins are foundational biomolecules of life playing a crucial role in a myriad of biological processes. Their function often requires interplay with other biomolecules, including proteins themselves. Protein-protein interactions (PPIs) are essential for ...

The field of protein design has made remarkable progress over the past decade. Historically, the low reliability of purely structure-based design methods limited their application, but recent strategies that combine structure-based and sequence-based calcu ...

Bacteria are ubiquitous single cellular organisms. Compared to eukaryotic cells, bacteria have two unique characteristics: the membrane-less nucleoid and the cell wall built of peptidoglycan (PG). In most bacteria, a single circular chromosome is compacted ...

Cyclic peptides are fascinating molecules abundantly found in nature and exploited as molecular format for drug development as well as other applications, ranging from research tools to food additives. Advances in peptide technologies made over many years ...

This article explores the escalating impact of natural disasters, particularly droughts, in the Southern African Development Community (SADC), with a specific focus on Eswatini. Over the last century, approximately 63 million people in SADC countries have ...

Connectivity between the hyporheic zone and the flow is essential for the development of benthos and the reproductive success of spawning fish. The infiltration of fine sediment leads to clogging of the riverbed, reducing porosity and vertical water exchan ...

High throughput wide-field second harmonic imaging enables the label-free imaging of interfacial (< 3 nm thick) water, with a spatial resolution of similar to 370 nm using similar to 100 ms acquisition times per image. The obtained interfacial orientationa ...

The rapid progress in the field of deep learning has had a significant impact on protein design. Deep learning methods have recently produced a breakthrough in protein structure prediction, leading to the availability of high-quality models for millions of ...

As the fundamental machinery orchestrating cellular functions, proteins influence the state of every cell profoundly. As cells exhibit significant variations from one to another, analyzing the proteome on a single-cell level is imperative to unravel their ...

This study combined protein modeling methods to generate the prolamins' fractions as precise as possible. Hence, gliadins, zeins, kafirins, hordeins, secalins, avenins and oryzins were generated based on their characteristics and disulfide mapping. Finding ...

Physical interactions between proteins are essential for most biological processes governing life. However, the molecular determinants of such interactions have been challenging to understand, even as genomic, proteomic and structural data increase. This k ...

Visualizing Biochemical Activities in Living Cells through Chemistry

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The effect of hydration and dynamics on the mass density of single proteins

Computational design of complex protein folds and soluble analogs of membrane proteins

Structural basis of BAK sequestration by MCL-1 in apoptosis

Recording physiological history of cells with chemical labeling

HaloTag-Based Tools for Live-Cell Imaging and Elucidation of Organellar Redox Biology

From Sequence to Dynamics to Function: Computational Design of Allostery and Ligand Selectivity in G-Protein Coupled Receptors

Modulating Weak Protein-Protein Cross-Interactions by the Addition of Free Amino Acids at Millimolar Concentrations

Fungal mycelia: From innovative materials to promising products: Insights and challenges

Mechanisms and functions of protein S-acylation

Engineering novel protein interactions with therapeutic potential using deep learning-guided surface design

Opportunities and challenges in design and optimization of protein function

Resolving bacterial cell biology: from replisome dynamics to cell wall synthesis

Cyclic Peptides for Drug Development

Geospatial Tools and Remote Sensing Strategies for Timely Humanitarian Response: A Case Study on Drought Monitoring in Eswatini

Impact of substrate clogging on vertical connectivity

Quantified interface chemistry and biophysics via water imaging

A new age in protein design empowered by deep learning

Development of tools and methods for protein identification: From single molecules to in vivo applications

Prolamins' 3D structure: A new insight into protein modeling using the language of numbers and shapes

De novo design of protein interactions with learned surface fingerprints