Publication

Method of preparation of cdna library useful for efficient mrna sequencing and uses thereof

Bart Deplancke, Riccardo Dainese, Daniel Alpern, Mustafa Demir
2022
Patents

Abstract

The invention relates to methods for the preparation of method of preparation of cDNA library based on one or many RNA samples useful for efficient RNA sequencing and uses thereof. The invention further relates to related tools and kits useful in said method.

Official source

https://infoscience.epfl.ch/entities/publication/3cc39a93-b1d9-4216-a6d2-861c60629031

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Bart Deplancke, Riccardo Dainese, Daniel Alpern, Mustafa Demir
2022
Patents

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/3cc39a93-b1d9-4216-a6d2-861c60629031

About this result

Related concepts (17)

DNA sequencing

DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, DNA Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics.

Third-generation sequencing

Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods currently under active development. Third generation sequencing technologies have the capability to produce substantially longer reads than second generation sequencing, also known as next-generation sequencing. Such an advantage has critical implications for both genome science and the study of biology in general. However, third generation sequencing data have much higher error rates than previous technologies, which can complicate downstream genome assembly and analysis of the resulting data.

Clinical metagenomic sequencing

Clinical metagenomic next-generation sequencing (mNGS) is the comprehensive analysis of microbial and host genetic material (DNA or RNA) in clinical samples from patients by next-generation sequencing. It uses the techniques of metagenomics to identify and characterize the genome of bacteria, fungi, parasites, and viruses without the need for a prior knowledge of a specific pathogen directly from clinical specimens.

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