Versatile spectral modulation of a broadband source for digital holographic microscopy

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Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

Adrien Charles-François Raymond Descloux

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

Spectral Properties of the Signal in Phase-Sensitive Optical Time-Domain Reflectometry with Direct Detection

Luc Thévenaz, Marcelo Alfonso Soto Hernandez, Xin Lu, Li Zhang

The spectral properties of the Rayleigh backscattered traces measured by a phase-sensitive optical time-domain reflectometer (øOTDR) with direct detection are theoretically and experimentally analyzed. The spectrum of the measured øOTDR signal is found to ...

IEEE Institute of Electrical and Electronics Engineers2020

Near-field phase profile and far-field contrast modulation for micro-lens arrays at the refraction limit

Toralf Scharf, Maryam Yousefi, Daniel Necesal

We investigate the far-field pattern generation for a micro-lens array (MLA) illuminated under different conditions. Plane wave and Gaussian beam illumination are considered for an MLA with a small diameter of 27 microns and 30 microns period. At these dim ...

2020

Aliasing mitigation in optical microscopy of dynamic biological samples by use of temporally modulated color illumination and a standard RGB camera

Michael Stefan Daniel Liebling, Christian Jaques

Significance: Despite recent developments in microscopy, temporal aliasing can arise when imaging dynamic samples. Modern sampling frameworks, such as generalized sampling, mitigate aliasing but require measurement of temporally overlapping and potentially ...

SPIE Society of Photo-optical Instrumentation Engineers2020

Optical phase contrast imaging of human retinal cells by changing the tissue refractive index

Christophe Moser, Mathieu Künzi, Timothé Laforest, Laura Emmanuelle Kowalczuk

Purpose : Based on oblique partially coherent illumination of transparent samples, we developed a simple custom Optical Phase Imaging (OPI) microscope providing a label-free, semi-quantitative phase contrast imaging. The aim of this study was to explore th ...

ASSOC RESEARCH VISION OPHTHALMOLOGY INC2020

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

3D nanometrology of transparent objects by phase calibration of a basic bright-field microscope for multiple illumination apertures

Martinus Gijs, Daniel Migliozzi, Bingying Zhao

Optical retrieval of the structure of transparent objects at the nanoscale requires adapted techniques capable of probing their interaction with light. Here, we considered a method based on calibration of the defocusing with partially coherent illumination ...

2020

Broadband quasi-phase-matching in dispersion-engineered all-optically poled silicon nitride waveguides

Rui Ning Wang, Tobias Kippenberg, Camille Sophie Brès, Junqiu Liu, Edgars Nitiss, Ozan Yakar, Boris Zabelich

Quasi-phase-matching (QPM) has become one of the most common approaches for increasing the efficiency of nonlinear three-wave mixing processes in integrated photonic circuits. Here, we provide a study of dispersion engineering of QPM second-harmonic (SH) g ...

2020

Reconfigurable radiofrequency filters based on versatile soliton microcombs

Tobias Kippenberg, Camille Sophie Brès, Maxim Karpov, Junqiu Liu, Jianqi Hu, Arslan Sajid Raja, Anton Lukashchuk, Jijun He

The rapidly maturing integrated Kerr microcombs show significant potential for microwave photonics. Yet, state-of-the-art microcomb-based radiofrequency filters have required programmable pulse shapers, which inevitably increase the system cost, footprint, ...

Nature Research2020

Controlling free electrons with optical whispering-gallery modes

Tobias Kippenberg

Free-electron beams are versatile probes of microscopic structure and composition(1,2), and have revolutionized atomic-scale imaging in several fields, from solid-state physics to structural biology(3). Over the past decade, the manipulation and interactio ...

NATURE PUBLISHING GROUP2020

Label-free, scalable and point-of-care imaging platform for rapid analysis of biomarker

Hatice Altug, Alexander Belushkin, Filiz Yesilköy, Yasaman Jahani

We propose a disruptive point-of-care (PoC) imaging platform based on lens-free interference phase-contrast imaging for rapid detection of biomarker such as for sepsis and potentially other diseases (e.g. cancer). It enables simultaneous analysis of potent ...

SPIE-INT SOC OPTICAL ENGINEERING2019

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