Publication

Chemical synthesis and functional characterization of melittin-TASP molecules

Horst Arthur Vogel
1994
Conference Papers

Abstract

A symposium report. The total synthesis of melittin-TASP, T4-(4a26) (TASP = template assembled synthetic proteins) is described together with its structural and ion-channel forming properties in planar lipid bilayers. A voltage-dependent channel activity is obsd. upon application of pos. voltage on the side of protein addn. This rectification is indicative of four helixes structurally forced into an unidirectional orientation within the channel aggregate. Melittin-TASPs show a two orders of magnitude higher potency in permeabilizing the bilayer membranes than native melittin. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/3684b8b0-69e1-4914-a348-9ee704bcad90

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Horst Arthur Vogel
1994
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/3684b8b0-69e1-4914-a348-9ee704bcad90

About this result

Related concepts (32)

Lipid bilayer

The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around all cells. The cell membranes of almost all organisms and many viruses are made of a lipid bilayer, as are the nuclear membrane surrounding the cell nucleus, and membranes of the membrane-bound organelles in the cell. The lipid bilayer is the barrier that keeps ions, proteins and other molecules where they are needed and prevents them from diffusing into areas where they should not be.

Voltage-gated ion channel

Voltage-gated ion channels are a class of transmembrane proteins that form ion channels that are activated by changes in the electrical membrane potential near the channel. The membrane potential alters the conformation of the channel proteins, regulating their opening and closing. Cell membranes are generally impermeable to ions, thus they must diffuse through the membrane through transmembrane protein channels. They have a crucial role in excitable cells such as neuronal and muscle tissues, allowing a rapid and co-ordinated depolarization in response to triggering voltage change.

Lipid bilayer phase behavior

One property of a lipid bilayer is the relative mobility (fluidity) of the individual lipid molecules and how this mobility changes with temperature. This response is known as the phase behavior of the bilayer. Broadly, at a given temperature a lipid bilayer can exist in either a liquid or a solid phase. The solid phase is commonly referred to as a “gel” phase. All lipids have a characteristic temperature at which they undergo a transition (melt) from the gel to liquid phase.

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Chemical synthesis and functional characterization of melittin-TASP molecules

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