Publication

Intelligent Pinhole with Sub-Micrometer Resolution

Thomas Sidler, Sébastian Favre
2004
Conference Papers

Abstract

An intelligent pinhole for confocal microscopy consisting of four position controlled blades forming a rectangular aperture from 3x3μm to 500 x 500 μm was built. This aperture can be positioned without drift under computer control in a positioning range with a sub-micrometer precision. Each blade is suspended by an elastic hinges and moved by a linear magnetic actuator. An integrated position transducer with a reproducibility of better than 40 nm allows a settling time of less than 75 ms. The whole pinhole system is small sized (40x40x20 mm) with an electrical consumption of less than 2W. A first application in a scanning confocal microscope demonstrated the usefulness of such a micro-opto-mechanical system.

Official source

https://infoscience.epfl.ch/entities/publication/34930d1c-ad39-4d02-987b-ed333157e4c9

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Thomas Sidler, Sébastian Favre
2004
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/34930d1c-ad39-4d02-987b-ed333157e4c9

About this result

Related concepts (29)

Microscope

A microscope () is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisible to the eye unless aided by a microscope. There are many types of microscopes, and they may be grouped in different ways.

Confocal microscopy

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures (a process known as optical sectioning) within an object.

Super-resolution microscopy

Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy as well as those that use the Pendry Superlens and near field scanning optical microscopy) or on the far-field.

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Intelligent Pinhole with Sub-Micrometer Resolution

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