Publication

Delivery of antisense oligonucleotides from photopolymerized hydrogels

1993
Conference Papers

Abstract

Antisense oligonucleotides promise to be highly potent therapeutic agents. Nanomolar extracellular concns. of phosphorothioate oligonucleotides have been reported to significantly alter gene expression in cultured cells. (Tidd, 1990). PEG derived hydrogels appear to be appropriate for delivering these concns. or higher for prolonged periods of time, and should protect the antisense oligonucleotides from enzymic degrdn. prior to cellular uptake. Release from these hydrogels follows predictable and alterable kinetics. The addnl. ability to polymerize the oligonucleotide-contg. macromer soln. in situ makes this advancement particularly exciting. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/339a8b71-ce6a-4bec-9c54-475b98c88944

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1993
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/339a8b71-ce6a-4bec-9c54-475b98c88944

About this result

Related concepts (20)

Antisense RNA

Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript (NAT) or antisense oligonucleotide, is a single stranded RNA that is complementary to a protein coding messenger RNA (mRNA) with which it hybridizes, and thereby blocks its translation into protein. The asRNAs (which occur naturally) have been found in both prokaryotes and eukaryotes, and can be classified into short (200 nucleotides) non-coding RNAs (ncRNAs).

Oligonucleotide

Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics. Commonly made in the laboratory by solid-phase chemical synthesis, these small bits of nucleic acids can be manufactured as single-stranded molecules with any user-specified sequence, and so are vital for artificial gene synthesis, polymerase chain reaction (PCR), DNA sequencing, molecular cloning and as molecular probes.

Gene expression

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA.

Related publications (1)

Delivery of antisense oligonucleotides from photopolymerized hydrogels

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