Publication

Covalent labeling of fusion proteins with chemical probes in living cells

Horst Arthur Vogel, Kai Johnsson, Horst Pick
2003
Journal Articles

Abstract

A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl group from O6-alkylguanine-DNA to one of its cysteine residues. Labeling is possible in bacterial as well as eukaryotic cells and is independent of the nature of the label, thereby opening up new ways to study proteins in vivo. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/31d42e06-ba6f-4cb1-bb99-43eb233974f2

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Horst Arthur Vogel, Kai Johnsson, Horst Pick
2003
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/31d42e06-ba6f-4cb1-bb99-43eb233974f2

About this result

Related concepts (32)

Cell (biology)

The cell is the basic structural and functional unit of all forms of life. Every cell consists of cytoplasm enclosed within a membrane, and contains many macromolecules such as proteins, DNA and RNA, as well as many small molecules of nutrients and metabolites. The term comes from the Latin word cellula meaning 'small room'. Cells can acquire specified function and carry out various tasks within the cell such as replication, DNA repair, protein synthesis, and motility. Cells are capable of specialization and mobility within the cell.

Fusion protein

Fusion proteins or chimeric (kī-ˈmir-ik) proteins (literally, made of parts from different sources) are proteins created through the joining of two or more genes that originally coded for separate proteins. Translation of this fusion gene results in a single or multiple polypeptides with functional properties derived from each of the original proteins. Recombinant fusion proteins are created artificially by recombinant DNA technology for use in biological research or therapeutics.

Eukaryote

The eukaryotes constitute the domain of Eukaryota (juːˈkærioʊts,_-əts), organisms whose cells have a nucleus. All animals, plants, fungi, and many unicellular organisms are eukaryotes. They constitute a major group of life forms, alongside the two groups of prokaryotes, the Bacteria and the Archaea. Eukaryotes represent a small minority of the number of organisms, but due to their generally much larger size, their collective global biomass is much larger than that of prokaryotes.

Related publications (8)

Covalent labeling of fusion proteins with chemical probes in living cells

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