Quantitative Holographic Imaging for Single-Cell Phenotyping in a Microfluidic System

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The early establishment of a reservoir of latently infected T cells is a sobering obstacle to HIV eradication, in spite of the efficacy of current antiretroviral therapies. That latent proviruses might also hide in multipotent hematopoietic stem cells suggests an even more formidable challenge and potentially has therapeutic implications.

In this work, we analyze the effect of rough ZnO front electrodes developed in-house favoring high current versus "smoother" substrates favoring good Voc and FF on the properties of microcrystalline silicon (μc-Si:H) cells. Complete μc-Si:H p-i-n solar cells with intrinsic layer thicknesses varying from 1 to 6μm were deposited using very high frequency plasma-enhanced chemical vapor deposition. The better scattering capabilities of rough substrates induce a significantly higher cell current in comparison to

The efficiency of on-chip microfluidic liquid-liquid extraction was improved by two orders of magnitude by spraying one liquid in another compared to the state-of-the-H-filters. We are developing a portable, integrated optical microsystem in the mid-infrared range to detect cocaine in human saliva. To ensure a good signal-to-noise ratio, the analyte needs to be transferred to an IR-transparent organic solvent by liquid-liquid extraction for subsequent on-chip analysis by IR-spectroscopy. In microfluidics, l

Digital Holographic Microscopy (DHM) has been used to investigate the spontaneous cell membrane fluctuations (CMF) of the Red Blood Cell. DHM as an interferometric technique is able to accurately provide the wavefront deformation induced by a transparent specimen, including living cells in a transmission configuration. From a numerical reconstruction of a single hologram, quantitative phase contrast images are obtained. The local phase shift is proportional to the specimen thickness with accuracy of 5-10 nm

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic Microscopy (DHM) and widefield epifluorescence microscopy modes in a time sequential basis. DHM provides information on real time cellular morphology. EGFP transfected actin filaments in mouse myoblast cells function as the reporter for the fluorescence microscopy mode. Experimental setup is fixed in the RPM to observe microgravity

Microglia are the resident immune cells of the central nervous system (CNS). Microglia undergo rapid activation in response to even minor pathological changes in the CNS. The activation state of microglia and tissue macrophages is characterized by two extremes that are known as M1 and M2. M1 is triggered upon pro-inflammatory stimuli and lead to the secretion of molecules involved in brain defense but also in neurotoxicity. M2 arises upon anti-inflammatory stimuli and is hallmarked by the secretion of molec

By enhancing the environment of micro electrode arrays with microfluidic channels we cultured primary neuronal cells in patterned two- and three dimensional structures. Alternatively, the same microfluidic channels can be used to generate a local chemical stimulation over a two-dimensional neuronal cell culture. We demonstrate that a significantly different of spontaneous activity pattern was recorded from the same neuronal cell culture under perfusion of 4.5μM bicuculline at 20 nl/s and 200 nl/s.

Synthetic bioactive hydrogels have been widely recognized as key elements of emerging strategies to engineer tissues. However, the current shortage of highly specific and biocompatible methods to form and functionalize these materials hampers their wide pharmaceutical and medical use. In particular, enzymatic reactions are underexplored for the synthesis of bioactive hydrogels. Here, we present an approach by which phosphopantetheinyl transferase (PPTase), a small (16.2 kDa) enzyme that plays a key role in

RNA interference has become an increasingly important tool for all aspects of molecular biology. Nevertheless, there is a technological challenge for performing gene knock-down with chemical transfection agents, as many relevant cell types are refractory to efficient transport of short interfering RNA (siRNA) into cytosol using these classical carrier-based methods. The aim of this project was to evaluate the potential of electroporation as a generic transfection method and to compare it with a standard and

Cardiac diseases represent the major cause of mortality in the Western world. The discovery of the promising human Cardiac Adherent Proliferating (CAP) cells for a potential cardiac cell therapy by the Tissue Engineering group induced the need of a reliable animal model. The aim of the project is to identify a porcine cardiac cell population having similar characteristics as human CAP cells. Here a protocol for isolating cells from porcine heart biopsies have been established, as well as the in vitro expans

A novel imaging method employing the different kinetics of the energetic states of fluorescent markers is presented, where the contrast depends on the triplet kinetics. An attractive feature of triplet states is their long lifetime, rendering them highly sensitive to the immediate environment of their fluorescent host molecules. They typically have a lifetime of the order of ~10us to 10ms, which is 3-6 orders of magnitude longer than the lifetimes of fluorescent singlet states. The concept exploits characte

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We have recently proposed a new and efficient decoupling procedure allowing to directly obtain separate measureme

Quantitative Holographic Imaging for Single-Cell Phenotyping in a Microfluidic System

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Profaning the Ultimate Sanctuary: HIV Latency in Hematopoietic Stem Cells

Substrate dependent stability and interplay between optical and electrical properties in μc-Si:H single junction solar cells

Improved method for liquid-liquid extraction on low-cost polymer chips

Exploring red blood cell membrane dynamics with digital holographic microscopy

Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Establishment of polarized microglial cells derived from mouse embryonic stem cells

Engineering the micro electrode environment with microfluidics: A new approach for cell culture patterning or controlled chemical stimulation

Phosphopantetheinyl transferase-catalyzed formation of bioactive hydrogels for tissue engineering

Cellular transfection of small interfering ribonucleic acids. Electroporation as an alternative to liposome-based delivery transfection methods

Isolation and characterisation of a porcine cardiac adherent cell population from heart biopsies

Triplet imaging contrast by modulated excitation

Erythrocytes volume and refractive index measurement with a digital holographic microscope