Publication

ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

Philipp Bucher, Giovanna Ambrosini
2011
Journal Articles

Abstract

Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.

Official source

https://infoscience.epfl.ch/entities/publication/304630ca-8605-41be-ab93-7b405f8f3775

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ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

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