Publication

Development of carbon balances for continuous animal cell cultures using 14C-labeled glucose

Urs von Stockar
1997
Conference Papers

Abstract

Continuous cultures of a hybridoma cell line (Zac3) were performed in a stirred tank reactor with protein-free media. At steady state the medium was replaced by one contg. U-14C-labeled glucose. The fluxes leaving the reactor were analyzed with a scintillation counter to det. the specific activity of each. The gas and solid phases allowed detn. of the oxidn. and the assimilation ratio of the consumed glucose. The liq. phase was sepd. chromatog. to det. quant. the different species consumed or formed by the cells. Identical expts. were also performed with U-14C glutamine labeled. The major part of the glucose consumed (83.65%) was converted to lactate. By this method it was possible to complete the carbon balance. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/2155ace2-c6ce-4ace-a621-b76b3b299901

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Urs von Stockar
1997
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/2155ace2-c6ce-4ace-a621-b76b3b299901

About this result

Related concepts (32)

Glucose

Glucose is a sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, using energy from sunlight, where it is used to make cellulose in cell walls, the most abundant carbohydrate in the world. In energy metabolism, glucose is the most important source of energy in all organisms. Glucose for metabolism is stored as a polymer, in plants mainly as starch and amylopectin, and in animals as glycogen.

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

Glucose 6-phosphate

Glucose 6-phosphate (G6P, sometimes called the Robison ester) is a glucose sugar phosphorylated at the hydroxy group on carbon 6. This dianion is very common in cells as the majority of glucose entering a cell will become phosphorylated in this way. Because of its prominent position in cellular chemistry, glucose 6-phosphate has many possible fates within the cell. It lies at the start of two major metabolic pathways: glycolysis and the pentose phosphate pathway.

Related publications (32)

Development of carbon balances for continuous animal cell cultures using 14C-labeled glucose

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