Publication

Production of immunoglobulin A in different reactor configurations

Urs von Stockar, Ian William Marison
1995
Journal Articles

Abstract

A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metab. and performances for IgA prodn. Cultures in T-flask and batch bioreactor were found to be glutamine-limited. Ammonia and lactate were produced in significant amts. IgA productivity was found to be const. and growth assocd. Final IgA concn. was similar in both systems. In fed-batch cultures, supplemented with glutamine and glucose, max. viable cell concn. was increased by 60% and final IgA concn. by 155%. The hollow fiber reactor was able to produce very large amts. of IgA at very high concns., similar to the value found in ascites fluid. The productivity of Zac3 is similar to the values reported for IgG-producing cell lines. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/18032fdb-84c1-4c2f-9737-a6eda91f9c4b

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Urs von Stockar, Ian William Marison
1995
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/18032fdb-84c1-4c2f-9737-a6eda91f9c4b

About this result

Related concepts (20)

Antibody

An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the pathogen, called an antigen. Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (analogous to a key) on an antigen, allowing these two structures to bind together with precision.

Ascites

Ascites is the abnormal build-up of fluid in the abdomen. Technically, it is more than 25 ml of fluid in the peritoneal cavity, although volumes greater than one liter may occur. Symptoms may include increased abdominal size, increased weight, abdominal discomfort, and shortness of breath. Complications can include spontaneous bacterial peritonitis. In the developed world, the most common cause is liver cirrhosis. Other causes include cancer, heart failure, tuberculosis, pancreatitis, and blockage of the hepatic vein.

Monoclonal antibody

A monoclonal antibody (mAb, more rarely called moAb) is an antibody produced from a cell lineage made by cloning a unique white blood cell. All subsequent antibodies derived this way trace back to a unique parent cell. Monoclonal antibodies can have monovalent affinity, binding only to the same epitope (the part of an antigen that is recognized by the antibody). In contrast, polyclonal antibodies bind to multiple epitopes and are usually made by several different antibody-secreting plasma cell lineages.