Publication

Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR

Stewart Cole
2008
Journal Articles

Abstract

BACKGROUND: Phi29 polymerase based amplification methods provides amplified DNA with minimal changes in sequence and relative abundance for many biomedical applications. RNA virus detection using microarrays, however, can present a challenge because phi29 DNA polymerase cannot amplify RNA nor small cDNA fragments (

Official source

https://infoscience.epfl.ch/entities/publication/16bffad8-eaf1-4df4-9109-1053b17faebe

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Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR

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