A microfluidic platform for whole-membrane integrity profiling in live neuronal cells

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Carbon membranes yielding high selectivity as well as high permeance are attractive to advance the membrane-based gas separation. Herein, we report ultrathin carbon membranes (UCMs) which deliver enhanced gas separation performance through oxygen-modulated pyrolysis of poly(4-vinylpyridine) precursor. We show that O 2 in pyrolysis environment, transforms the otherwise uniform carbon network featuring a ~ 3.9 Å characteristic interlayer spacing into disrupted UCMs (d-UCMs). These d-UCMs possess a multimodal

Ether glycerophospholipids bear a long chain alcohol attached via an alkyl or vinyl ether bond at the sn1 position of the glycerol backbone. Ether lipids play a significant role in physiology and human health. However, their cellular functions remain largely unknown due to a lack of tools for identifying their subcellular localization and interacting proteins. Here, we address this methodological gap by synthesizing minimally modified bifunctional ether lipid probes by introducing diazirine and alkyne group

Diabetes mellitus is a globally growing disease with more than 180 million cases worldwide and no cure exists. In order to minimize the many medical complications, tight glucose monitoring has been shown to be the best alternative. Many diabetics require i ...

In order to realize an implantable glucose sensing system designed within this project, the realization of a selective nanoporous membrane is essential. The final goal of this sub-project is establishing a fabrication process of a semi-permeable membrane consisting of nanoporous layer that has a function to allow diffusion of glucose while ensuring retention of larger molecules.

Dense planar and tubular oxygen separation membranes of La 0.6Ca0.4Fe0.75Co0.25O3-d were investigated as reactors for the partial oxidation (POX) of methane to syngas. Disk-shaped membranes were prepared by compaction and tubes by extrusion. Their permeation properties were measured in an air/argon pO2 gradient as a function of temperature. At 900°C, the oxygen flux through a 1.26 mm thick membrane was 0.075 micromol/cm2s and through a 0.25 mm thick tube, 0.24 micromol/cm2s. For the POX measurements, a cata

A novel in situ product removal (ISPR) method that uses microcapsules to ext. inhibitory products from the reaction suspension is introduced into fermn. technol. More specifically, L-phenylalanine (L-phe) was transformed by Saccharomyces cerevisiae to 2-phenylethanol (PEA), which is inhibitory toward the yeast. In order to continuously remove PEA from the vicinity of the cells, the reaction suspension was brought into contact with capsules of 2.2-mm diam. that had a hydrophobic core of di-Bu sebacate and an

What is the meaning' of a single spike? Spike-triggered averaging (reverse correlations') yields the typical input just before a spike. Similarly, cross-correlations describe the probability of firing an output spike given (one additional) presynaptic input spike. In this paper we analytically calculate reverse and cross-correlations for a spiking neuron model with escape noise. The influence of neuronal parameters (such as the membrane time constant, the noise level, and the mean firing rate) on the form

A review with 130 refs. on optical techniques such as total internal reflection fluorescence, waveguides, and SPR for the detection of mol. interactions of membrane receptors. The outlook on how these emerging techniques may be applied to solve important questions in the field of mol. membrane biol. is given. [on SciFinder (R)]

A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled receptor NK2. A fluorescent unnatural amino acid was introduced at known sites into NK2 by suppression of nonsense codons with the aid of a chem. misacylated synthetic tRNA specifically designed for the incorporation of unnatural amino acids during expression in

A new class of lipid mols. is synthesized, based on two dipalmitoylphosphatidic mols., each extended at the lipid phosphate by a hydrophilic spacer chain of ethoxy groups of variable length, which are then coupled as a bilipid via a terminal disulfide group at the hydrophilic spacer. These anchor-bearing "thiolipids" can attach to gold substrates by forming stable gold-sulfur bonds. In this way, the authors can couple lipid bilayers to gold surfaces, with the possibility of preserving a water layer betwee

A microfluidic platform for whole-membrane integrity profiling in live neuronal cells

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Oxygen-induced multimodal ultramicroporous structure in 10-nm-thick carbon membranes for enhanced hydrogen separation

Bifunctional Probes Reveal the Rules of Intracellular Ether Lipid Transport

Molecular selective interface for an implantable glucose sensor based on the viscosity variation of a sensitive fluid containing dextran and Concanavalin A

Implantable Glucose Sensor, IGLUS III – Membrane Technology –

Planar and Tubular perovskite-type membrane reactors for the partial oxidation of methane to syngas

xxvel type of in situ extraction: Use of solvent containing microcapsules for the bioconversion of 2-phenylethanol from L-phenylalanine by Saccharomyces cerevisiae

Coding properties of spiking neurons: reverse- and cross-correlations

Emerging techniques for investigating molecular interactions at lipid membranes

Probing ligand-receptor recognition in G protein-coupled receptors through biosynthetic incorporation of fluorescent amino acids at specific sites and measurement of distances by fluorescence energy transfer

A new class of thiolipids for the attachment of lipid bilayers on gold surfaces